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Preparing recombinant gonad organ cultures.

Publication ,  Journal Article
Capel, B; Batchvarov, J
Published in: CSH Protoc
November 1, 2008

INTRODUCTIONIt can be useful to assay migration between any two adjacent tissues during development. This protocol assays cell migration between the gonad and mesonephros using tissue recombination between genetically marked and unmarked tissue, combined with an organ culture technique. First, agar blocks are prepared in a custom-built mold. The size and shape of the wells are important to maintain the authentic three-dimensional morphology of the organ; the molds here are designed specifically to accommodate the gonad/mesonephros complex. Freshly dissected organs are then transferred to grooves within the agar blocks, where they are allowed to grow over 24-48 h. Using this protocol, organs develop with good morphology, and show only an ~12-h delay relative to in vivo development.

Duke Scholars

Published In

CSH Protoc

DOI

Publication Date

November 1, 2008

Volume

2008

Start / End Page

pdb.prot5078

Location

United States

Related Subject Headings

  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)
 

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Capel, B., & Batchvarov, J. (2008). Preparing recombinant gonad organ cultures. CSH Protoc, 2008, pdb.prot5078. https://doi.org/10.1101/pdb.prot5078
Capel, Blanche, and Jordan Batchvarov. “Preparing recombinant gonad organ cultures.CSH Protoc 2008 (November 1, 2008): pdb.prot5078. https://doi.org/10.1101/pdb.prot5078.
Capel B, Batchvarov J. Preparing recombinant gonad organ cultures. CSH Protoc. 2008 Nov 1;2008:pdb.prot5078.
Capel, Blanche, and Jordan Batchvarov. “Preparing recombinant gonad organ cultures.CSH Protoc, vol. 2008, Nov. 2008, p. pdb.prot5078. Pubmed, doi:10.1101/pdb.prot5078.
Capel B, Batchvarov J. Preparing recombinant gonad organ cultures. CSH Protoc. 2008 Nov 1;2008:pdb.prot5078.

Published In

CSH Protoc

DOI

Publication Date

November 1, 2008

Volume

2008

Start / End Page

pdb.prot5078

Location

United States

Related Subject Headings

  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0306 Physical Chemistry (incl. Structural)