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Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam.

Publication ,  Journal Article
Tang, H; Ross, A; Capel, B
Published in: Dev Biol
June 1, 2008

In the testis, the continuous production of sperm is maintained by a small population of stem cells called germ line stem cells (GSCs) in Drosophila, or spermatogonial stem cells (SSCs) in mammals. This stem cell population can self-renew or produce daughter cells that differentiate into mature sperm. In Drosophila, BMP signals inhibit GSC differentiation by blocking transcription of the gene bag of marbles (bam). Once bam is activated, germ cells initiate differentiation. We identified a novel gene in mouse, Gm114, that shows homology to Drosophila bam. In male germ cells, expression of Gm114 begins at 12.5-13.5 days post coitum (dpc), the stage in mice when germ cells cease proliferation and begin differentiation into prospermatogonia. In the adult testis, Gm114 is highly expressed in differentiated spermatocytes and spermatids but not in undifferentiated spermatogonia, strongly suggesting that, similar to Bam, GM114 plays an important role in mammalian germ line stem cell self-renewal and differentiation. Interestingly, deletion of the majority of the GM114 protein does not affect mouse viability or fertility. This suggests that either there is a function for the remaining N-terminal of GM114, or that there are alternative mechanisms in the mammalian system that control germ cell differentiation.

Duke Scholars

Published In

Dev Biol

DOI

EISSN

1095-564X

Publication Date

June 1, 2008

Volume

318

Issue

1

Start / End Page

73 / 81

Location

United States

Related Subject Headings

  • Testis
  • Sequence Alignment
  • Proteins
  • Phylogeny
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Male
  • Humans
  • Germ Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tang, H., Ross, A., & Capel, B. (2008). Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. Dev Biol, 318(1), 73–81. https://doi.org/10.1016/j.ydbio.2008.03.001
Tang, Hao, Andrea Ross, and Blanche Capel. “Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam.Dev Biol 318, no. 1 (June 1, 2008): 73–81. https://doi.org/10.1016/j.ydbio.2008.03.001.
Tang H, Ross A, Capel B. Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. Dev Biol. 2008 Jun 1;318(1):73–81.
Tang, Hao, et al. “Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam.Dev Biol, vol. 318, no. 1, June 2008, pp. 73–81. Pubmed, doi:10.1016/j.ydbio.2008.03.001.
Tang H, Ross A, Capel B. Expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. Dev Biol. 2008 Jun 1;318(1):73–81.
Journal cover image

Published In

Dev Biol

DOI

EISSN

1095-564X

Publication Date

June 1, 2008

Volume

318

Issue

1

Start / End Page

73 / 81

Location

United States

Related Subject Headings

  • Testis
  • Sequence Alignment
  • Proteins
  • Phylogeny
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Male
  • Humans
  • Germ Cells