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Notch signaling maintains Leydig progenitor cells in the mouse testis.

Publication ,  Journal Article
Tang, H; Brennan, J; Karl, J; Hamada, Y; Raetzman, L; Capel, B
Published in: Development
November 2008

During testis development, fetal Leydig cells increase their population from a pool of progenitor cells rather than from proliferation of a differentiated cell population. However, the mechanism that regulates Leydig stem cell self-renewal and differentiation is unknown. Here, we show that blocking Notch signaling, by inhibiting gamma-secretase activity or deleting the downstream target gene Hairy/Enhancer-of-split 1, results in an increase in Leydig cells in the testis. By contrast, constitutively active Notch signaling in gonadal somatic progenitor cells causes a dramatic Leydig cell loss, associated with an increase in undifferentiated mesenchymal cells. These results indicate that active Notch signaling restricts fetal Leydig cell differentiation by promoting a progenitor cell fate. Germ cell loss and abnormal testis cord formation were observed in both gain- and loss-of-function gonads, suggesting that regulation of the Leydig/interstitial cell population is important for male germ cell survival and testis cord formation.

Duke Scholars

Published In

Development

DOI

ISSN

0950-1991

Publication Date

November 2008

Volume

135

Issue

22

Start / End Page

3745 / 3753

Location

England

Related Subject Headings

  • Transcription Factor HES-1
  • Stem Cells
  • Signal Transduction
  • Receptors, Notch
  • Mice, Transgenic
  • Mice
  • Male
  • Leydig Cells
  • Homeodomain Proteins
  • Gene Expression Regulation, Developmental
 

Citation

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Tang, H., Brennan, J., Karl, J., Hamada, Y., Raetzman, L., & Capel, B. (2008). Notch signaling maintains Leydig progenitor cells in the mouse testis. Development, 135(22), 3745–3753. https://doi.org/10.1242/dev.024786
Tang, Hao, Jennifer Brennan, Jeannie Karl, Yoshio Hamada, Lori Raetzman, and Blanche Capel. “Notch signaling maintains Leydig progenitor cells in the mouse testis.Development 135, no. 22 (November 2008): 3745–53. https://doi.org/10.1242/dev.024786.
Tang H, Brennan J, Karl J, Hamada Y, Raetzman L, Capel B. Notch signaling maintains Leydig progenitor cells in the mouse testis. Development. 2008 Nov;135(22):3745–53.
Tang, Hao, et al. “Notch signaling maintains Leydig progenitor cells in the mouse testis.Development, vol. 135, no. 22, Nov. 2008, pp. 3745–53. Pubmed, doi:10.1242/dev.024786.
Tang H, Brennan J, Karl J, Hamada Y, Raetzman L, Capel B. Notch signaling maintains Leydig progenitor cells in the mouse testis. Development. 2008 Nov;135(22):3745–3753.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

November 2008

Volume

135

Issue

22

Start / End Page

3745 / 3753

Location

England

Related Subject Headings

  • Transcription Factor HES-1
  • Stem Cells
  • Signal Transduction
  • Receptors, Notch
  • Mice, Transgenic
  • Mice
  • Male
  • Leydig Cells
  • Homeodomain Proteins
  • Gene Expression Regulation, Developmental