Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene.

Publication ,  Journal Article
Mill, J; Dempster, E; Caspi, A; Williams, B; Moffitt, T; Craig, I
Published in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
June 2006

Monozygotic (MZ) twin concordance for a range of psychiatric conditions is rarely 100%. It has been suggested that epigenetic factors, such as DNA methylation, may account for a proportion of the variation in behavioral traits observed between these genetically identical individuals. In this study we have quantitatively assessed the methylation status of two CpG sites in the promoter region of the COMT gene in 12 MZ twins-pairs discordant for birth weight, but otherwise clinically unaffected. DNA was obtained at age 5-years using buccal swabs, and modified using sodium-bisulfite treatment. Methylation profiles were assessed using Pyrosequencing, a technology enabling the precise degree of methylation to be assessed at any CpG site. We found that the degree of methylation at the two CpG sites was highly correlated, but there was considerable variation in the concordance of methylation levels between MZ twin-pairs. Some MZ twin-pairs showed a high degree of methylation concordance, whereas others differed markedly in their methylation profiles. Such epigenetic variation between genetically identical individuals may play a key role in the etiology of psychopathology, and explain the incomplete phenotypic concordance observed in MZ twins.

Duke Scholars

Published In

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics

DOI

EISSN

1552-485X

ISSN

1552-4841

Publication Date

June 2006

Volume

141B

Issue

4

Start / End Page

421 / 425

Related Subject Headings

  • Twins, Monozygotic
  • Promoter Regions, Genetic
  • Male
  • Humans
  • Female
  • DNA Methylation
  • DNA
  • CpG Islands
  • Child, Preschool
  • Catechol O-Methyltransferase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mill, J., Dempster, E., Caspi, A., Williams, B., Moffitt, T., & Craig, I. (2006). Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene. American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : The Official Publication of the International Society of Psychiatric Genetics, 141B(4), 421–425. https://doi.org/10.1002/ajmg.b.30316
Mill, Jonathan, Emma Dempster, Avshalom Caspi, Benjamin Williams, Terrie Moffitt, and Ian Craig. “Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene.American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : The Official Publication of the International Society of Psychiatric Genetics 141B, no. 4 (June 2006): 421–25. https://doi.org/10.1002/ajmg.b.30316.
Mill J, Dempster E, Caspi A, Williams B, Moffitt T, Craig I. Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene. American journal of medical genetics Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics. 2006 Jun;141B(4):421–5.
Mill, Jonathan, et al. “Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene.American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : The Official Publication of the International Society of Psychiatric Genetics, vol. 141B, no. 4, June 2006, pp. 421–25. Epmc, doi:10.1002/ajmg.b.30316.
Mill J, Dempster E, Caspi A, Williams B, Moffitt T, Craig I. Evidence for monozygotic twin (MZ) discordance in methylation level at two CpG sites in the promoter region of the catechol-O-methyltransferase (COMT) gene. American journal of medical genetics Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics. 2006 Jun;141B(4):421–425.
Journal cover image

Published In

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics

DOI

EISSN

1552-485X

ISSN

1552-4841

Publication Date

June 2006

Volume

141B

Issue

4

Start / End Page

421 / 425

Related Subject Headings

  • Twins, Monozygotic
  • Promoter Regions, Genetic
  • Male
  • Humans
  • Female
  • DNA Methylation
  • DNA
  • CpG Islands
  • Child, Preschool
  • Catechol O-Methyltransferase