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Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells.

Publication ,  Journal Article
Palmer, JM; Chen, BJ; DeOliveira, D; Le, N-D; Chao, NJ
Published in: Bone Marrow Transplant
February 2010

Rapamycin (RAPA) is an immunosuppressive drug that prevents and treats graft-versus-host disease (GVHD) after allogeneic hematopoietic cell transplant (HCT). One possible mechanism for its efficacy is induction of tolerance, through increased number or enhanced survival of regulatory T cells. In our experiments, B10.D2 BM and splenocytes were injected into lethally irradiated BALB/cJ recipients. The mice received i.p. injections of either RAPA or vehicle control on days 1-28. There was a significant survival advantage in RAPA-treated mice. Evaluation of the skin biopsies showed a dense cellular infiltrate in RAPA-treated mice. Further characterization of these cells revealed a higher percentage of regulatory T cells characterized by FoxP3-positive cells in high-dose RAPA-treated mice as compared with controls on day 30. This effect appears to be dose dependent. When peripheral blood analysis for FoxP3-positive cells was performed, there was no significant difference observed in the RAPA-treated mice as compared with control mice. These data show a novel mechanism of rapamycin in GVHD, accumulation of regulatory T cells in the GVHD target tissue: the skin.

Duke Scholars

Published In

Bone Marrow Transplant

DOI

EISSN

1476-5365

Publication Date

February 2010

Volume

45

Issue

2

Start / End Page

379 / 384

Location

England

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Skin
  • Sirolimus
  • Mice, Inbred BALB C
  • Mice
  • Liver
  • Intestines
  • Immunology
  • Graft vs Host Disease
  • Forkhead Transcription Factors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Palmer, J. M., Chen, B. J., DeOliveira, D., Le, N.-D., & Chao, N. J. (2010). Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells. Bone Marrow Transplant, 45(2), 379–384. https://doi.org/10.1038/bmt.2009.140
Palmer, J. M., B. J. Chen, D. DeOliveira, N. -. D. Le, and N. J. Chao. “Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells.Bone Marrow Transplant 45, no. 2 (February 2010): 379–84. https://doi.org/10.1038/bmt.2009.140.
Palmer JM, Chen BJ, DeOliveira D, Le N-D, Chao NJ. Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells. Bone Marrow Transplant. 2010 Feb;45(2):379–84.
Palmer, J. M., et al. “Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells.Bone Marrow Transplant, vol. 45, no. 2, Feb. 2010, pp. 379–84. Pubmed, doi:10.1038/bmt.2009.140.
Palmer JM, Chen BJ, DeOliveira D, Le N-D, Chao NJ. Novel mechanism of rapamycin in GVHD: increase in interstitial regulatory T cells. Bone Marrow Transplant. 2010 Feb;45(2):379–384.

Published In

Bone Marrow Transplant

DOI

EISSN

1476-5365

Publication Date

February 2010

Volume

45

Issue

2

Start / End Page

379 / 384

Location

England

Related Subject Headings

  • T-Lymphocytes, Regulatory
  • Skin
  • Sirolimus
  • Mice, Inbred BALB C
  • Mice
  • Liver
  • Intestines
  • Immunology
  • Graft vs Host Disease
  • Forkhead Transcription Factors