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Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice.

Publication ,  Journal Article
Chute, JP; Muramoto, GG; Salter, AB; Meadows, SK; Rickman, DW; Chen, B; Himburg, HA; Chao, NJ
Published in: Blood
March 15, 2007

Flk-1(+) endothelial progenitors contribute critically to the definitive onset of hematopoiesis during embryogenesis. Recent studies have suggested that adult sources of endothelial cells also possess hematopoietic activity. In this study, we sought to determine whether transplantation of primary vascular endothelial cells (ECs) could enhance the hematopoietic recovery and survival of irradiated mice. C57Bl6 mice were exposed to sublethal and lethal doses of irradiation and were subsequently given transplants of either primary murine brain-derived ECs (MBECs) or fetal blood-derived ECs (FBECs). Mice that received a transplant with MBECs alone demonstrated accelerated BM cellular recovery, radioprotection of BM c-kit(+)sca-1(-)lin(-) progenitors and enhanced regeneration of c-kit(+)sca-1(+)lin(-) (KSL) stem/progenitor cells following irradiation compared with controls. MBEC transplantation also facilitated the recovery of circulating white blood cell and platelet counts following radiation exposure. Remarkably, 57% of mice that received a transplant with MBECs alone survived long term following 1050 cGy exposure, which was 100% lethal in control mice. FBEC transplantation was also associated with increased survival compared with controls, although these mice did not survive in the long term. These data suggest that reestablishment of endothelial cell activity can improve the hematopoietic recovery and survival of irradiated mice.

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Published In

Blood

DOI

ISSN

0006-4971

Publication Date

March 15, 2007

Volume

109

Issue

6

Start / End Page

2365 / 2372

Location

United States

Related Subject Headings

  • Survival Rate
  • Stem Cells
  • Mice
  • Immunology
  • Hematopoiesis
  • Gene Expression
  • Endothelial Cells
  • Cytokines
  • Cells, Cultured
  • Cell Transplantation
 

Citation

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Chute, J. P., Muramoto, G. G., Salter, A. B., Meadows, S. K., Rickman, D. W., Chen, B., … Chao, N. J. (2007). Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice. Blood, 109(6), 2365–2372. https://doi.org/10.1182/blood-2006-05-022640
Chute, John P., Garrett G. Muramoto, Alice B. Salter, Sarah K. Meadows, Dennis W. Rickman, Benny Chen, Heather A. Himburg, and Nelson J. Chao. “Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice.Blood 109, no. 6 (March 15, 2007): 2365–72. https://doi.org/10.1182/blood-2006-05-022640.
Chute JP, Muramoto GG, Salter AB, Meadows SK, Rickman DW, Chen B, et al. Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice. Blood. 2007 Mar 15;109(6):2365–72.
Chute, John P., et al. “Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice.Blood, vol. 109, no. 6, Mar. 2007, pp. 2365–72. Pubmed, doi:10.1182/blood-2006-05-022640.
Chute JP, Muramoto GG, Salter AB, Meadows SK, Rickman DW, Chen B, Himburg HA, Chao NJ. Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice. Blood. 2007 Mar 15;109(6):2365–2372.

Published In

Blood

DOI

ISSN

0006-4971

Publication Date

March 15, 2007

Volume

109

Issue

6

Start / End Page

2365 / 2372

Location

United States

Related Subject Headings

  • Survival Rate
  • Stem Cells
  • Mice
  • Immunology
  • Hematopoiesis
  • Gene Expression
  • Endothelial Cells
  • Cytokines
  • Cells, Cultured
  • Cell Transplantation