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Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury.

Publication ,  Journal Article
Muramoto, GG; Chen, B; Cui, X; Chao, NJ; Chute, JP
Published in: Biol Blood Marrow Transplant
May 2006

The risk of terrorism with nuclear or radiologic weapons is considered to be high over the coming decade. Ionizing radiation can cause a spectrum of hematologic toxicities, from mild myelosuppression to myeloablation and death. However, the potential regenerative capacity of human hematopoietic stem cells (HSCs) after radiation injury has not been well characterized. In this study, we sought to characterize the effects of ionizing radiation on human HSCs and to determine whether signals from vascular endothelial cells could promote the repair of irradiated HSCs. Exposure of human bone marrow CD34+ cells to 400 cGy caused a precipitous decline in hematopoietic progenitor cell content and primitive cells capable of repopulating nonobese diabetic/severe combined immunodeficient mice (SCID-repopulating cells), which was not retrievable via treatment with cytokines. Conversely, culture of 400 cGy-irradiated bone marrow CD34+ cells with endothelial cells under noncontact conditions supported the differential recovery of both viable progenitor cells and primitive SCID-repopulating cells. These data illustrate that vascular endothelial cells produce soluble factors that promote the repair and functional recovery of HSCs after radiation injury and suggest that novel factors with radiotherapeutic potential can be identified within this milieu.

Duke Scholars

Published In

Biol Blood Marrow Transplant

DOI

ISSN

1083-8791

Publication Date

May 2006

Volume

12

Issue

5

Start / End Page

530 / 540

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radiation Injuries
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Immunophenotyping
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
 

Citation

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ICMJE
MLA
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Muramoto, G. G., Chen, B., Cui, X., Chao, N. J., & Chute, J. P. (2006). Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury. Biol Blood Marrow Transplant, 12(5), 530–540. https://doi.org/10.1016/j.bbmt.2005.12.039
Muramoto, Garrett G., Benny Chen, Xiuyu Cui, Nelson J. Chao, and John P. Chute. “Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury.Biol Blood Marrow Transplant 12, no. 5 (May 2006): 530–40. https://doi.org/10.1016/j.bbmt.2005.12.039.
Muramoto GG, Chen B, Cui X, Chao NJ, Chute JP. Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury. Biol Blood Marrow Transplant. 2006 May;12(5):530–40.
Muramoto, Garrett G., et al. “Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury.Biol Blood Marrow Transplant, vol. 12, no. 5, May 2006, pp. 530–40. Pubmed, doi:10.1016/j.bbmt.2005.12.039.
Muramoto GG, Chen B, Cui X, Chao NJ, Chute JP. Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury. Biol Blood Marrow Transplant. 2006 May;12(5):530–540.
Journal cover image

Published In

Biol Blood Marrow Transplant

DOI

ISSN

1083-8791

Publication Date

May 2006

Volume

12

Issue

5

Start / End Page

530 / 540

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radiation Injuries
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Immunophenotyping
  • Immunology
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation