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Fusions of elastin-like polypeptides to pharmaceutical proteins.

Publication ,  Journal Article
Hassouneh, W; MacEwan, SR; Chilkoti, A
Published in: Methods in enzymology
January 2012

Elastin-like polypeptides (ELPs) are a class of stimulus-responsive biopolymers whose physicochemical properties and biocompatibility are particularly suitable for in vivo applications, such as drug delivery and tissue engineering. The lower critical solution temperature (LCST) behavior of ELPs allows them to be utilized as soluble macromolecules below their LCST, or as self-assembled nanoscale particles such as micelles, micron-scale coacervates, or viscous gels above their LCST, depending on the ELP architecture. As each ELP sequence is specified at its genetic level, functionalization of an ELP with peptides and proteins is simple to accomplish by the fusion of a gene encoding an ELP with that of the peptide or protein of interest. Protein ELP fusions, where the appended protein serves a therapeutic or targeting function, are suitable for applications in which the ELP can improve the systemic pharmacokinetics and biodistribution of the protein, or can be used as an injectable depot for sustained, local protein delivery. Here we describe considerations in the design of therapeutic protein ELP fusions and provide details of their gene design, expression, and purification.

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Published In

Methods in enzymology

DOI

EISSN

1557-7988

ISSN

0076-6879

Publication Date

January 2012

Volume

502

Start / End Page

215 / 237

Related Subject Headings

  • Transformation, Bacterial
  • Temperature
  • Recombinant Fusion Proteins
  • Protein Structure, Tertiary
  • Plasmids
  • Phase Transition
  • Peptides
  • Nephelometry and Turbidimetry
  • Molecular Sequence Data
  • Micelles
 

Citation

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Hassouneh, W., MacEwan, S. R., & Chilkoti, A. (2012). Fusions of elastin-like polypeptides to pharmaceutical proteins. Methods in Enzymology, 502, 215–237. https://doi.org/10.1016/b978-0-12-416039-2.00024-0
Hassouneh, Wafa, Sarah R. MacEwan, and Ashutosh Chilkoti. “Fusions of elastin-like polypeptides to pharmaceutical proteins.Methods in Enzymology 502 (January 2012): 215–37. https://doi.org/10.1016/b978-0-12-416039-2.00024-0.
Hassouneh W, MacEwan SR, Chilkoti A. Fusions of elastin-like polypeptides to pharmaceutical proteins. Methods in enzymology. 2012 Jan;502:215–37.
Hassouneh, Wafa, et al. “Fusions of elastin-like polypeptides to pharmaceutical proteins.Methods in Enzymology, vol. 502, Jan. 2012, pp. 215–37. Epmc, doi:10.1016/b978-0-12-416039-2.00024-0.
Hassouneh W, MacEwan SR, Chilkoti A. Fusions of elastin-like polypeptides to pharmaceutical proteins. Methods in enzymology. 2012 Jan;502:215–237.

Published In

Methods in enzymology

DOI

EISSN

1557-7988

ISSN

0076-6879

Publication Date

January 2012

Volume

502

Start / End Page

215 / 237

Related Subject Headings

  • Transformation, Bacterial
  • Temperature
  • Recombinant Fusion Proteins
  • Protein Structure, Tertiary
  • Plasmids
  • Phase Transition
  • Peptides
  • Nephelometry and Turbidimetry
  • Molecular Sequence Data
  • Micelles