Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Dual-order snapshot spectral imaging of plasmonic nanoparticles.

Publication ,  Journal Article
Nusz, GJ; Marinakos, SM; Rangarajan, S; Chilkoti, A
Published in: Applied optics
July 2011

The development of truly scalable, multiplexed optical microarrays requires a detection platform capable of simultaneous detection of multiple signals in real-time. We present a technique we term dual-order snapshot spectroscopic imaging (DOSSI) and demonstrate that it can be effectively used to collect spectrally resolved images of a full field of view of sparsely located spots in real time. Resonant peaks of plasmonic gold nanoparticles were tracked as a function of their surrounding refractive index. Measurement uncertainty analysis indicated that the spectral resolution of DOSSI in the described configuration is approximately 0.95 nm. Further, real-time measurements by DOSSI allowed discrimination between optically identical nanoparticles that were functionalized with two homologous small molecule ligands that bound to the same protein, albeit with different affinity, based purely on their different molecular interaction kinetics-a feat not possible with slower raster-type hyperspectral imaging systems, or other dark-field optical detection systems that solely rely on end point measurements. Kinetic measurements of plasmon bands by DOSSI can be performed with a relatively simple optical system, thereby opening up the possibility of developing low-cost detectors for arrayed plasmonic diagnostics.

Duke Scholars

Published In

Applied optics

DOI

EISSN

1539-4522

ISSN

1559-128X

Publication Date

July 2011

Volume

50

Issue

21

Start / End Page

4198 / 4206

Related Subject Headings

  • Surface Plasmon Resonance
  • Streptavidin
  • Protein Binding
  • Optics
  • Optical Phenomena
  • Nanotubes
  • Nanotechnology
  • Metal Nanoparticles
  • Gold
  • Biotin
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nusz, G. J., Marinakos, S. M., Rangarajan, S., & Chilkoti, A. (2011). Dual-order snapshot spectral imaging of plasmonic nanoparticles. Applied Optics, 50(21), 4198–4206. https://doi.org/10.1364/ao.50.004198
Nusz, Gregory J., Stella M. Marinakos, Srinath Rangarajan, and Ashutosh Chilkoti. “Dual-order snapshot spectral imaging of plasmonic nanoparticles.Applied Optics 50, no. 21 (July 2011): 4198–4206. https://doi.org/10.1364/ao.50.004198.
Nusz GJ, Marinakos SM, Rangarajan S, Chilkoti A. Dual-order snapshot spectral imaging of plasmonic nanoparticles. Applied optics. 2011 Jul;50(21):4198–206.
Nusz, Gregory J., et al. “Dual-order snapshot spectral imaging of plasmonic nanoparticles.Applied Optics, vol. 50, no. 21, July 2011, pp. 4198–206. Epmc, doi:10.1364/ao.50.004198.
Nusz GJ, Marinakos SM, Rangarajan S, Chilkoti A. Dual-order snapshot spectral imaging of plasmonic nanoparticles. Applied optics. 2011 Jul;50(21):4198–4206.

Published In

Applied optics

DOI

EISSN

1539-4522

ISSN

1559-128X

Publication Date

July 2011

Volume

50

Issue

21

Start / End Page

4198 / 4206

Related Subject Headings

  • Surface Plasmon Resonance
  • Streptavidin
  • Protein Binding
  • Optics
  • Optical Phenomena
  • Nanotubes
  • Nanotechnology
  • Metal Nanoparticles
  • Gold
  • Biotin