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Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.

Publication ,  Journal Article
Alang Ahmad, S; Hucknall, A; Chilkoti, A; Leggett, GJ
Published in: Langmuir : the ACS journal of surfaces and colloids
June 2010

The UV photodegradation of protein-resistant poly(oligo(ethylene glycol) methacrylate) (POEGMA) bottle-brush films, grown on silicon oxide by surface-initiated atom radical transfer polymerization, was studied using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Exposure to light with a wavelength of 244 nm caused a loss of polyether units from the brush structure and the creation of aldehyde groups that could be derivatized with amines. An increase was measured in the coefficient of friction of the photodegraded polymer brush compared to the native brush, attributed to the creation of a heterogeneous surface film, leading to increased energy dissipation through film deformation and the creation of new polar functional groups at the surface. Exposure of the films through a photomask yielded sharp, well-defined patterns. Analysis of topographical images showed that physical removal of material occurred during exposure, at a rate of 1.35 nm J(-1) cm(2). Using fluorescence microscopy, the adsorption of labeled proteins onto the exposed surfaces was studied. It was found that protein strongly adsorbed to exposed areas, while the masked regions retained their protein resistance. Exposure of the film to UV light from a scanning near-field optical microscope yielded submicrometer-scale patterns. These data indicate that a simple, rapid, one-step photoconversion of the poly(OEGMA) brush occurs that transforms it from a highly protein-resistant material to one that adsorbs protein and can covalently bind amine-containing molecules and that this photoconversion can be spatially addressed with high spatial resolution.

Duke Scholars

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

June 2010

Volume

26

Issue

12

Start / End Page

9937 / 9942

Related Subject Headings

  • Ultraviolet Rays
  • Proteins
  • Polyethylene Glycols
  • Photolysis
  • Molecular Imprinting
  • Chemical Physics
  • Amines
 

Citation

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ICMJE
MLA
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Alang Ahmad, S., Hucknall, A., Chilkoti, A., & Leggett, G. J. (2010). Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir : The ACS Journal of Surfaces and Colloids, 26(12), 9937–9942. https://doi.org/10.1021/la100438d
Alang Ahmad, Shahrul, Angus Hucknall, Ashutosh Chilkoti, and Graham J. Leggett. “Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.Langmuir : The ACS Journal of Surfaces and Colloids 26, no. 12 (June 2010): 9937–42. https://doi.org/10.1021/la100438d.
Alang Ahmad S, Hucknall A, Chilkoti A, Leggett GJ. Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir : the ACS journal of surfaces and colloids. 2010 Jun;26(12):9937–42.
Alang Ahmad, Shahrul, et al. “Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes.Langmuir : The ACS Journal of Surfaces and Colloids, vol. 26, no. 12, June 2010, pp. 9937–42. Epmc, doi:10.1021/la100438d.
Alang Ahmad S, Hucknall A, Chilkoti A, Leggett GJ. Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir : the ACS journal of surfaces and colloids. 2010 Jun;26(12):9937–9942.
Journal cover image

Published In

Langmuir : the ACS journal of surfaces and colloids

DOI

EISSN

1520-5827

ISSN

0743-7463

Publication Date

June 2010

Volume

26

Issue

12

Start / End Page

9937 / 9942

Related Subject Headings

  • Ultraviolet Rays
  • Proteins
  • Polyethylene Glycols
  • Photolysis
  • Molecular Imprinting
  • Chemical Physics
  • Amines