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Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair.

Publication ,  Journal Article
Betre, H; Setton, LA; Meyer, DE; Chilkoti, A
Published in: Biomacromolecules
September 2002

Elastin-like polypeptides (ELPs) are artificial polypeptides with unique properties that make them attractive as a biomaterial for tissue-engineered cartilage repair. ELPs are composed of a pentapeptide repeat, Val-Pro-Gly-Xaa-Gly (Xaa is any amino acid except Pro), that undergo an inverse temperature phase transition. They are soluble in aqueous solution below their transition temperature (T(t)) but aggregate when the solution temperature is raised above their T(t). This study investigates the rheological behavior of an un-cross-linked ELP, below and above its T(t), and also examines the ability of ELP to promote chondrogenesis in vitro. A thermally responsive ELP with a T(t) of 35 degrees C was synthesized using recombinant DNA techniques. The complex shear modulus of the ELP increased by 3 orders of magnitude as it underwent its inverse temperature phase transition, forming a coacervate, or gel-like, ELP phase. Values for the complex shear moduli of the un-cross-linked ELP coacervate are comparable to those reported previously for collagen, hyaluronan, and cross-linked synthetic hydrogels. Cell culture studies show that chondrocytes cultured in ELP coacervate maintain a rounded morphology and their chondrocytic phenotype, characterized by the synthesis of a significant amount of extracellular matrix composed of sulfated glycosaminoglycans and collagen. These results suggest that ELPs demonstrate great potential for use as in situ forming scaffolds for cartilaginous tissue repair.

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

September 2002

Volume

3

Issue

5

Start / End Page

910 / 916

Related Subject Headings

  • Tissue Engineering
  • Temperature
  • Swine
  • Rheology
  • Polymers
  • Peptides
  • Molecular Sequence Data
  • Genetic Engineering
  • Elastin
  • Chondrogenesis
 

Citation

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ICMJE
MLA
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Betre, H., Setton, L. A., Meyer, D. E., & Chilkoti, A. (2002). Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules, 3(5), 910–916. https://doi.org/10.1021/bm0255037
Betre, Helawe, Lori A. Setton, Dan E. Meyer, and Ashutosh Chilkoti. “Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair.Biomacromolecules 3, no. 5 (September 2002): 910–16. https://doi.org/10.1021/bm0255037.
Betre H, Setton LA, Meyer DE, Chilkoti A. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules. 2002 Sep;3(5):910–6.
Betre, Helawe, et al. “Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair.Biomacromolecules, vol. 3, no. 5, Sept. 2002, pp. 910–16. Epmc, doi:10.1021/bm0255037.
Betre H, Setton LA, Meyer DE, Chilkoti A. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules. 2002 Sep;3(5):910–916.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

September 2002

Volume

3

Issue

5

Start / End Page

910 / 916

Related Subject Headings

  • Tissue Engineering
  • Temperature
  • Swine
  • Rheology
  • Polymers
  • Peptides
  • Molecular Sequence Data
  • Genetic Engineering
  • Elastin
  • Chondrogenesis