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Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein

Publication ,  Journal Article
Shimoboji, T; Stayton, PS; Long, C; Chilkoti, A; Chen, G; Harris, JM; Hoffman, AS
Published in: Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium
December 1, 1996

A novel method for sensitive environmental control of the molecular recognition process between a protein receptor and its ligand is presented. The method is based on the conjugation of a smart, temperature-sensitive polymer, poly(N-isopropylacrylamide), to a specific genetically-engineered site, thiol. It was shown that the site-specific conjugation of a temperature-sensitive polymer to a genetically engineered protein provides a very sensitive temperature control of the ligand-protein recognition process. Such molecular switches could have widespread applications in medicine and biotechnology.

Duke Scholars

Published In

Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium

Publication Date

December 1, 1996

Volume

1

Start / End Page

897
 

Citation

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ICMJE
MLA
NLM
Shimoboji, T., Stayton, P. S., Long, C., Chilkoti, A., Chen, G., Harris, J. M., & Hoffman, A. S. (1996). Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Transactions of the Annual Meeting of the Society for Biomaterials in Conjunction with the International Biomaterials Symposium, 1, 897.
Shimoboji, T., P. S. Stayton, C. Long, A. Chilkoti, G. Chen, J. M. Harris, and A. S. Hoffman. “Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.” Transactions of the Annual Meeting of the Society for Biomaterials in Conjunction with the International Biomaterials Symposium 1 (December 1, 1996): 897.
Shimoboji T, Stayton PS, Long C, Chilkoti A, Chen G, Harris JM, et al. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. 1996 Dec 1;1:897.
Shimoboji, T., et al. “Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.” Transactions of the Annual Meeting of the Society for Biomaterials in Conjunction with the International Biomaterials Symposium, vol. 1, Dec. 1996, p. 897.
Shimoboji T, Stayton PS, Long C, Chilkoti A, Chen G, Harris JM, Hoffman AS. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. 1996 Dec 1;1:897.

Published In

Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium

Publication Date

December 1, 1996

Volume

1

Start / End Page

897