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Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines.

Publication ,  Journal Article
Woost, PG; Kolb, RJ; Finesilver, M; Mackraj, I; Imboden, H; Coffman, TM; Hopfer, U
Published in: In Vitro Cell Dev Biol Anim
2006

In the proximal convoluted tubule (PCT) angiotensin II (Ang II) modulates fluid and electrolyte transport through at least two pharmacologically distinct receptor subtypes: AT(1) and AT(2). Development of cell lines that lack these receptors are potentially useful models to probe the complex cellular details of Ang II regulation. To this end, angiotensin receptor- deficient mice were bred with an Immortomouse(R), which harbors a thermolabile SV40 large-T antigen (Tag). S1 PCT segments from kidneys of F(2) mice were microdissected, placed in culture, and maintained under conditions that enhanced cell growth, i.e., promoted Tag expression and thermostability. Three different types of angiotensin receptor-deficient cell lines, (AT(1A) [-/-], Tag [+/-]), (AT(1B) [-/-], Tag [+/-]), and (AT(1A) [-/-], AT(1B) [-/-], Tag [+/+]), as well as wild type cell lines were generated. Screening and characterization, which were conducted under culture conditions that promoted cellular differentiation, included: measurements of transepithelial transport, such as basal monolayer short-circuit current (Isc; -3 to 3 microA/cm2), basal monolayer conductance (G, 2 to 10 mS/cm2), Na3(+)-phosphate cotransport (DeltaIsc of 2 to 3 microA/cm(2) at 1 mM), and Na(3)(+)-succinate cotransport (DeltaIsc of 1 to 9 microA/cm(2) at 2 mM). Morphology of cell monolayers showed an extensive brush border, well-defined tight junctions, and primary cilia. Receptor functionality was assessed by Ang II-stimulated beta-arrestin 2 translocation and showed an Ang II-mediated response in wild type but not (AT(1A) [-/ -], AT(1B) [-/-]) cells. Cell lines were amplified, yielding a virtually unlimited supply of highly differentiated, transport-competent, angiotensin receptor-deficient PCT cell lines.

Duke Scholars

Published In

In Vitro Cell Dev Biol Anim

DOI

ISSN

1071-2690

Publication Date

2006

Volume

42

Issue

7

Start / End Page

189 / 200

Location

Germany

Related Subject Headings

  • beta-Arrestins
  • beta-Arrestin 2
  • Receptors, Angiotensin
  • RNA, Messenger
  • Mutation
  • Mice
  • Kidney Tubules, Proximal
  • Immunohistochemistry
  • Genotype
  • Electrolytes
 

Citation

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Woost, P. G., Kolb, R. J., Finesilver, M., Mackraj, I., Imboden, H., Coffman, T. M., & Hopfer, U. (2006). Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines. In Vitro Cell Dev Biol Anim, 42(7), 189–200. https://doi.org/10.1290/0511076.1
Woost, Philip G., Robert J. Kolb, Margaret Finesilver, Irene Mackraj, Hans Imboden, Thomas M. Coffman, and Ulrich Hopfer. “Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines.In Vitro Cell Dev Biol Anim 42, no. 7 (2006): 189–200. https://doi.org/10.1290/0511076.1.
Woost PG, Kolb RJ, Finesilver M, Mackraj I, Imboden H, Coffman TM, et al. Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines. In Vitro Cell Dev Biol Anim. 2006;42(7):189–200.
Woost, Philip G., et al. “Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines.In Vitro Cell Dev Biol Anim, vol. 42, no. 7, 2006, pp. 189–200. Pubmed, doi:10.1290/0511076.1.
Woost PG, Kolb RJ, Finesilver M, Mackraj I, Imboden H, Coffman TM, Hopfer U. Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines. In Vitro Cell Dev Biol Anim. 2006;42(7):189–200.
Journal cover image

Published In

In Vitro Cell Dev Biol Anim

DOI

ISSN

1071-2690

Publication Date

2006

Volume

42

Issue

7

Start / End Page

189 / 200

Location

Germany

Related Subject Headings

  • beta-Arrestins
  • beta-Arrestin 2
  • Receptors, Angiotensin
  • RNA, Messenger
  • Mutation
  • Mice
  • Kidney Tubules, Proximal
  • Immunohistochemistry
  • Genotype
  • Electrolytes