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Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice.

Publication ,  Journal Article
Wagner, C; Vitzthum, H; Castrop, H; Schumacher, K; Bucher, M; Albertin, S; Coffman, TM; Arendshorst, WJ; Kurtz, A
Published in: Pflugers Arch
November 2003

Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression.

Duke Scholars

Published In

Pflugers Arch

DOI

ISSN

0031-6768

Publication Date

November 2003

Volume

447

Issue

2

Start / End Page

214 / 222

Location

Germany

Related Subject Headings

  • Ribonucleases
  • Renin
  • Ramipril
  • RNA, Messenger
  • Prostaglandin-Endoperoxide Synthases
  • Physiology
  • Nuclease Protection Assays
  • Mice, Inbred C57BL
  • Mice
  • Male
 

Citation

APA
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MLA
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Wagner, C., Vitzthum, H., Castrop, H., Schumacher, K., Bucher, M., Albertin, S., … Kurtz, A. (2003). Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice. Pflugers Arch, 447(2), 214–222. https://doi.org/10.1007/s00424-003-1157-1
Journal cover image

Published In

Pflugers Arch

DOI

ISSN

0031-6768

Publication Date

November 2003

Volume

447

Issue

2

Start / End Page

214 / 222

Location

Germany

Related Subject Headings

  • Ribonucleases
  • Renin
  • Ramipril
  • RNA, Messenger
  • Prostaglandin-Endoperoxide Synthases
  • Physiology
  • Nuclease Protection Assays
  • Mice, Inbred C57BL
  • Mice
  • Male