Scholars@Duke publication: H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection.

H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection.

Publication , Journal Article

Felix, NJ; Brickey, WJ; Griffiths, R; Zhang, J; Van Kaer, L; Coffman, T; Ting, JP

Published in: J Exp Med

July 3, 2000

The role played by antigenic peptides bound to major histocompatibility complex (MHC) molecules is evaluated with H2-DMalpha(-/)- mice. These mice have predominantly class II-associated invariant chain peptide (CLIP)-, not antigenic peptide-bound, MHC class II. H2-DMalpha(-/)- donor heart grafts survived three times longer than wild-type grafts and slightly longer than I-A(beta)(b)-(/)- grafts. Proliferative T cell response was absent, and cytolytic response was reduced against the H2-DMalpha(-/)- grafts in vivo. Residual cytolytic T cell and antibody responses against intact MHC class I lead to eventual rejection. Removal of both H2-DMalpha and beta2-microglobulin (beta2m) in cardiac grafts lead to greater (8-10 times) graft survival, whereas removal of beta2m alone did not have any effect. These results demonstrate the significance of peptide rather than just allogeneic MHC, in eliciting graft rejection.

Duke Scholars

Author Thomas Myron Coffman Medicine, Nephrology

Published In

J Exp Med

DOI

10.1084/jem.192.1.31

ISSN

0022-1007

Publication Date

July 3, 2000

Volume

192

Issue

Start / End Page

31 / 40

Location

United States

Related Subject Headings

Transplantation, Homologous
Th2 Cells
Th1 Cells
Myocardium
Mice, Knockout
Mice, Inbred Strains
Mice, Inbred DBA
Mice, Inbred BALB C
Mice
Major Histocompatibility Complex

Citation

APA

Chicago

ICMJE

MLA

NLM

Felix, N. J., Brickey, W. J., Griffiths, R., Zhang, J., Van Kaer, L., Coffman, T., & Ting, J. P. (2000). H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection. J Exp Med, 192(1), 31–40. https://doi.org/10.1084/jem.192.1.31

Felix, N. J., W. J. Brickey, R. Griffiths, J. Zhang, L. Van Kaer, T. Coffman, and J. P. Ting. “H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection.” J Exp Med 192, no. 1 (July 3, 2000): 31–40. https://doi.org/10.1084/jem.192.1.31.

Felix NJ, Brickey WJ, Griffiths R, Zhang J, Van Kaer L, Coffman T, et al. H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection. J Exp Med. 2000 Jul 3;192(1):31–40.

Felix, N. J., et al. “H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection.” J Exp Med, vol. 192, no. 1, July 2000, pp. 31–40. Pubmed, doi:10.1084/jem.192.1.31.

Felix NJ, Brickey WJ, Griffiths R, Zhang J, Van Kaer L, Coffman T, Ting JP. H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection. J Exp Med. 2000 Jul 3;192(1):31–40.