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Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells.

Publication ,  Journal Article
Tomlinson, RL; Abreu, EB; Ziegler, T; Ly, H; Counter, CM; Terns, RM; Terns, MP
Published in: Mol Biol Cell
September 2008

Telomere maintenance by telomerase is critical for the unlimited division potential of most human cancer cells. The two essential components of human telomerase, telomerase RNA (hTR) and telomerase reverse transcriptase (hTERT), are recruited from distinct subnuclear sites to telomeres during S phase. Throughout the remainder of the cell cycle hTR is found primarily in Cajal bodies. The localization of hTR to Cajal bodies and telomeres is specific to cancer cells where telomerase is active and is not observed in primary cells. Here we show that the trafficking of hTR to both telomeres and Cajal bodies depends on hTERT. RNA interference-mediated depletion of hTERT in cancer cells leads to loss of hTR from both Cajal bodies and telomeres without affecting hTR levels. In addition, expression of hTERT in telomerase-negative cells (including primary and ALT cancer cell lines) induces hTR to localize to both sites. Factors that did not stimulate hTR localization in our experiments include increased hTR RNA levels and Cajal body numbers, and expression of SV40 large T antigen and oncogenic Ras. Our findings suggest that the trafficking of telomerase to Cajal bodies and telomeres in cancer cells correlates with and depends on the assembly of the enzyme.

Duke Scholars

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

September 2008

Volume

19

Issue

9

Start / End Page

3793 / 3800

Location

United States

Related Subject Headings

  • Telomere
  • Telomerase
  • S Phase
  • RNA, Untranslated
  • RNA, Long Noncoding
  • RNA
  • Microscopy, Fluorescence
  • In Situ Hybridization, Fluorescence
  • Humans
  • Hela Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tomlinson, R. L., Abreu, E. B., Ziegler, T., Ly, H., Counter, C. M., Terns, R. M., & Terns, M. P. (2008). Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells. Mol Biol Cell, 19(9), 3793–3800. https://doi.org/10.1091/mbc.e08-02-0184
Tomlinson, Rebecca L., Eladio B. Abreu, Tania Ziegler, Hinh Ly, Christopher M. Counter, Rebecca M. Terns, and Michael P. Terns. “Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells.Mol Biol Cell 19, no. 9 (September 2008): 3793–3800. https://doi.org/10.1091/mbc.e08-02-0184.
Tomlinson RL, Abreu EB, Ziegler T, Ly H, Counter CM, Terns RM, et al. Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells. Mol Biol Cell. 2008 Sep;19(9):3793–800.
Tomlinson, Rebecca L., et al. “Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells.Mol Biol Cell, vol. 19, no. 9, Sept. 2008, pp. 3793–800. Pubmed, doi:10.1091/mbc.e08-02-0184.
Tomlinson RL, Abreu EB, Ziegler T, Ly H, Counter CM, Terns RM, Terns MP. Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells. Mol Biol Cell. 2008 Sep;19(9):3793–3800.

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

September 2008

Volume

19

Issue

9

Start / End Page

3793 / 3800

Location

United States

Related Subject Headings

  • Telomere
  • Telomerase
  • S Phase
  • RNA, Untranslated
  • RNA, Long Noncoding
  • RNA
  • Microscopy, Fluorescence
  • In Situ Hybridization, Fluorescence
  • Humans
  • Hela Cells