Skip to main content
Journal cover image

Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A.

Publication ,  Journal Article
Heymann, JJ; Gabricević, M; Mietzner, TA; Crumbliss, AL
Published in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
February 2010

The bacterial transferrin ferric binding protein A (FbpA) requires an exogenous anion to facilitate iron sequestration, and subsequently to shuttle the metal across the periplasm to the cytoplasmic membrane. In the diverse conditions of the periplasm, numerous anions are known to be present. Prior in vitro experiments have demonstrated the ability of multiple anions to fulfill the synergistic iron-binding requirement, and the identity of the bound anion has been shown to modulate important physicochemical properties of iron-bound FbpA (FeFbpA). Here we address the kinetics and mechanism of anion exchange for the FeFbpA-nitrilotriacetate (NTA) assembly with several biologically relevant anions (citrate, oxalate, phosphate, and pyrophosphate), with nonphysiologic NTA serving as a representative synergistic anion/chelator. The kinetic data are consistent with an anion-exchange process that occurs in multiple steps, dependent on the identity of both the entering anion and the leaving anion. The exchange mechanism may proceed either as a direct substitution or through an intermediate FeFbpA-X* assembly based on anion (X) identity. Our kinetic results further develop an understanding of exogenous anion lability in the periplasm, as well as address the final step of the iron-free FbpA (apo-FbpA)/Fe(3+) sequestration mechanism. Our results highlight the kinetic significance of the FbpA anion binding site, demonstrating a correlation between apo-FbpA/anion affinity and the FeFbpA rate of anion exchange, further supporting the requirement of an exogenous anion to complete tight sequestration of iron by FbpA, and developing a mechanism for anion exchange within FeFbpA that is dependent on the identity of both the entering anion and the leaving anion.

Duke Scholars

Published In

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry

DOI

EISSN

1432-1327

ISSN

0949-8257

Publication Date

February 2010

Volume

15

Issue

2

Start / End Page

237 / 248

Related Subject Headings

  • Phosphates
  • Periplasmic Binding Proteins
  • Periplasm
  • Oxalates
  • Nitrilotriacetic Acid
  • Neisseria gonorrhoeae
  • Kinetics
  • Iron-Binding Proteins
  • Citric Acid
  • Chromatography, Ion Exchange
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Heymann, J. J., Gabricević, M., Mietzner, T. A., & Crumbliss, A. L. (2010). Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A. Journal of Biological Inorganic Chemistry : JBIC : A Publication of the Society of Biological Inorganic Chemistry, 15(2), 237–248. https://doi.org/10.1007/s00775-009-0589-2
Heymann, Jared J., Mario Gabricević, Timothy A. Mietzner, and Alvin L. Crumbliss. “Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A.Journal of Biological Inorganic Chemistry : JBIC : A Publication of the Society of Biological Inorganic Chemistry 15, no. 2 (February 2010): 237–48. https://doi.org/10.1007/s00775-009-0589-2.
Heymann JJ, Gabricević M, Mietzner TA, Crumbliss AL. Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 2010 Feb;15(2):237–48.
Heymann, Jared J., et al. “Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A.Journal of Biological Inorganic Chemistry : JBIC : A Publication of the Society of Biological Inorganic Chemistry, vol. 15, no. 2, Feb. 2010, pp. 237–48. Epmc, doi:10.1007/s00775-009-0589-2.
Heymann JJ, Gabricević M, Mietzner TA, Crumbliss AL. Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 2010 Feb;15(2):237–248.
Journal cover image

Published In

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry

DOI

EISSN

1432-1327

ISSN

0949-8257

Publication Date

February 2010

Volume

15

Issue

2

Start / End Page

237 / 248

Related Subject Headings

  • Phosphates
  • Periplasmic Binding Proteins
  • Periplasm
  • Oxalates
  • Nitrilotriacetic Acid
  • Neisseria gonorrhoeae
  • Kinetics
  • Iron-Binding Proteins
  • Citric Acid
  • Chromatography, Ion Exchange