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Inter- and intrainstitutional evaluation of automated volumetric capillary cytometry for the quantitation of CD4- and CD8-positive T lymphocytes in the peripheral blood of persons infected with human immunodeficiency virus. Site Investigators and the NIAID New CD4 Technologies Focus Group.

Publication ,  Journal Article
O'Gorman, MR; Gelman, R
Published in: Clin Diagn Lab Immunol
March 1997

Volumetric capillary cytometry (VCC) is a new technology that involves the detection and enumeration of dually fluorochrome-labeled cells in a precise volume. We compared the accuracy and precision of VCC with the accuracy and precision of flow cytometry and hematology (F&H) for the measurement of the absolute numbers of CD4 and CD8 T cells in the whole blood of patients infected with human immunodeficiency virus. Five laboratories, each with a different F&H system and certified by the National institute of Allergy and Infectious Diseases flow cytometry proficiency testing program, were shipped aliquots of the same samples from a central site in addition to procuring samples locally. In general, the VCC technology generated CD4 and CD8 T-cell counts which were lower than those obtained with F&H. Intralaboratory variability of replicate CD4 T-cell determinations was similar for both technologies except in the local samples with CD4 counts less than 200/microliters, where the VCC variability was higher than the F&H variability. Interlaboratory variability on replicate CD4 T-cell counts made by VCC was significantly less than that when counts were made by F&H. The VCC instrument has automated CD4 and CD8 T-cell enumeration in whole blood and has consolidated the process to a single platform. Its performance in this evaluation indicates that it may represent a viable alternative to F&H for obtaining absolute T-cell subset counts.

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Published In

Clin Diagn Lab Immunol

DOI

ISSN

1071-412X

Publication Date

March 1997

Volume

4

Issue

2

Start / End Page

173 / 179

Location

United States

Related Subject Headings

  • Microbiology
  • Lymphocyte Count
  • Immunology
  • Humans
  • Hematology
  • HIV Infections
  • Flow Cytometry
  • Evaluation Studies as Topic
  • Cytophotometry
  • CD8-Positive T-Lymphocytes
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

Clin Diagn Lab Immunol

DOI

ISSN

1071-412X

Publication Date

March 1997

Volume

4

Issue

2

Start / End Page

173 / 179

Location

United States

Related Subject Headings

  • Microbiology
  • Lymphocyte Count
  • Immunology
  • Humans
  • Hematology
  • HIV Infections
  • Flow Cytometry
  • Evaluation Studies as Topic
  • Cytophotometry
  • CD8-Positive T-Lymphocytes