Skip to main content
Journal cover image

Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'.

Publication ,  Journal Article
Minning, DM; Gow, AJ; Bonaventura, J; Braun, R; Dewhirst, M; Goldberg, DE; Stamler, JS
Published in: Nature
September 30, 1999

The parasitic nematode Ascaris lumbricoides infects one billion people worldwide. Its perienteric fluid contains an octameric haemoglobin that binds oxygen nearly 25,000 times more tightly than does human haemoglobin. Despite numerous investigations, the biological function of this molecule has remained elusive. The distal haem pocket contains a metal, oxygen and thiol, all of which are known to be reactive with nitric oxide. Here we show that Ascaris haemoglobin enzymatically consumes oxygen in a reaction driven by nitric oxide, thus keeping the perienteric fluid hypoxic. The mechanism of this reaction involves unprecedented chemistry of a haem group, a thiol and nitric oxide. We propose that Ascaris haemoglobin functions as a 'deoxygenase', using nitric oxide to detoxify oxygen. The structural and functional adaptations of Ascaris haemoglobin suggest that the molecular evolution of haemoglobin can be rationalized by its nitric oxide related functions.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

September 30, 1999

Volume

401

Issue

6752

Start / End Page

497 / 502

Location

England

Related Subject Headings

  • Swine
  • Spectrum Analysis
  • S-Nitrosothiols
  • Oxygen
  • Oxidoreductases
  • Nitroso Compounds
  • Nitric Oxide
  • Mutagenesis
  • Ligands
  • Hemoglobins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Minning, D. M., Gow, A. J., Bonaventura, J., Braun, R., Dewhirst, M., Goldberg, D. E., & Stamler, J. S. (1999). Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'. Nature, 401(6752), 497–502. https://doi.org/10.1038/46822
Minning, D. M., A. J. Gow, J. Bonaventura, R. Braun, M. Dewhirst, D. E. Goldberg, and J. S. Stamler. “Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'.Nature 401, no. 6752 (September 30, 1999): 497–502. https://doi.org/10.1038/46822.
Minning DM, Gow AJ, Bonaventura J, Braun R, Dewhirst M, Goldberg DE, et al. Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'. Nature. 1999 Sep 30;401(6752):497–502.
Minning, D. M., et al. “Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'.Nature, vol. 401, no. 6752, Sept. 1999, pp. 497–502. Pubmed, doi:10.1038/46822.
Minning DM, Gow AJ, Bonaventura J, Braun R, Dewhirst M, Goldberg DE, Stamler JS. Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'. Nature. 1999 Sep 30;401(6752):497–502.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

September 30, 1999

Volume

401

Issue

6752

Start / End Page

497 / 502

Location

England

Related Subject Headings

  • Swine
  • Spectrum Analysis
  • S-Nitrosothiols
  • Oxygen
  • Oxidoreductases
  • Nitroso Compounds
  • Nitric Oxide
  • Mutagenesis
  • Ligands
  • Hemoglobins