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Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus).

Publication ,  Journal Article
Hasspieler, BM; Behar, JV; Di Giulio, RT
Published in: Ecotoxicology and environmental safety
June 1994

Glutathione-dependent defense against xenobiotic toxicity is a multifaceted phenomenon that has been well characterized in mammals. This study undertakes a comparison of two benthic fish species, the channel catfish and brown bullhead, in terms of characteristics of the glutathione system. The channel catfish, a species that has seldom been observed to express pollutant-mediated neoplasia in field studies, was observed to have significantly higher constitutive levels of hepatic total glutathione and reduced glutatione (GSH). Brown bullhead, a species that is often observed to express neoplasia in contaminated systems, had significantly higher hepatic levels of glutathione disulfide. Furthermore, catfish expressed higher levels of activity of the enzymes gamma-glutamylcysteine synthetase (GCS), glutathione reductase (GR), and glutathione S-transferase, whereas bullhead expressed higher hepatic glutathione peroxidase (GPOX) activity. Both species responded to treatment with the redox active quinone, menadione, by expressing elevated hepatic content of total glutathione. However, the induction response was more rapid and more extensive in catfish compared to that in bullhead. This is attributable to the observed interspecific difference in GCS activity. Following treatment with the organic peroxide, tert-butyl hydroperoxide (t-BOOH), bullhead hepatic glutathione was depleted up to 4 hr post-treatment, whereas catfish demonstrated no significant depletion of glutathione in response to t-BOOH. The differing responses to t-BOOH are attributable to interspecific differences in hepatic GPOX and GR activity. Bullhead, therefore, appear to be more susceptible to the effects of GSH arylators and oxidants based upon constitutive levels of glutathione, related enzyme activities, and the response of this system to model xenobiotics.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Ecotoxicology and environmental safety

DOI

EISSN

1090-2414

ISSN

0147-6513

Publication Date

June 1994

Volume

28

Issue

1

Start / End Page

82 / 90

Related Subject Headings

  • tert-Butylhydroperoxide
  • Vitamin K
  • Strategic, Defence & Security Studies
  • Species Specificity
  • Peroxides
  • Oxidoreductases
  • Liver
  • Ictaluridae
  • Glutathione
  • Fishes
 

Citation

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Chicago
ICMJE
MLA
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Hasspieler, B. M., Behar, J. V., & Di Giulio, R. T. (1994). Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicology and Environmental Safety, 28(1), 82–90. https://doi.org/10.1006/eesa.1994.1036
Hasspieler, B. M., J. V. Behar, and R. T. Di Giulio. “Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus).Ecotoxicology and Environmental Safety 28, no. 1 (June 1994): 82–90. https://doi.org/10.1006/eesa.1994.1036.
Hasspieler BM, Behar JV, Di Giulio RT. Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicology and environmental safety. 1994 Jun;28(1):82–90.
Hasspieler, B. M., et al. “Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus).Ecotoxicology and Environmental Safety, vol. 28, no. 1, June 1994, pp. 82–90. Epmc, doi:10.1006/eesa.1994.1036.
Hasspieler BM, Behar JV, Di Giulio RT. Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicology and environmental safety. 1994 Jun;28(1):82–90.
Journal cover image

Published In

Ecotoxicology and environmental safety

DOI

EISSN

1090-2414

ISSN

0147-6513

Publication Date

June 1994

Volume

28

Issue

1

Start / End Page

82 / 90

Related Subject Headings

  • tert-Butylhydroperoxide
  • Vitamin K
  • Strategic, Defence & Security Studies
  • Species Specificity
  • Peroxides
  • Oxidoreductases
  • Liver
  • Ictaluridae
  • Glutathione
  • Fishes