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Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile.

Publication ,  Journal Article
Kannangai, R; Diehl, AM; Sicklick, J; Rojkind, M; Thomas, D; Torbenson, M
Published in: Hum Pathol
April 2005

BACKGROUND AND AIMS: Angiomyolipomas (AMLs) of the liver are rare neoplasms composed of large epithelioid cells with intermixed fat and blood vessels. Hepatic AMLs have no clear normal-cell counterpart in the liver. However, AMLs and stellate cells both are positive for neural crest-derived markers including HMB-45 antigen. METHODS: To further explore the similarities between hepatic AMLs and stellate cells, gene expression of a hepatic AML was studied by cDNA microarray. Real-time polymerase chain reaction was used to confirm gene expression. Hepatic stellate cells can be quiescent, activated, or have a myofibroblastic phenotype depending on their state of activation. Expression of known markers of activated stellate cells was compared between the AML, activated primary mouse stellate cells, and stellate cell lines with activated and myofibroblastic phenotypes. Next, 5 novel genes from the AML were selected because they were not previously known to be markers of stellate cells and mRNA expression measured in the activated mouse stellate cells and in myofibroblastic stellate cell lines. Finally, expression levels of 10 novel genes were determined in 5 cirrhotic and 5 noncirrhotic human livers. RESULTS: Overexpression of known markers of activated stellate cells including transforming growth factor beta (TGF- beta ), smooth muscle actin, and collagen was found in the hepatic AML. Three of 5 novel markers that were identified in the AML, RRAD (Ras-related associated with diabetes), CTSK (cathepsin K), and NIBAN were also found to be overexpressed in activated stellate cells compared with quiescent or myofibroblastic stellate cells. In addition, 9 of 10 novel genes overexpressed in AML were also overexpressed in cirrhotic human livers versus noncirrhotic livers. CONCLUSIONS: Hepatic AMLs share a similar gene expression profile and may differentiate toward activated stellate cells.

Duke Scholars

Published In

Hum Pathol

DOI

ISSN

0046-8177

Publication Date

April 2005

Volume

36

Issue

4

Start / End Page

341 / 347

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Pathology
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Mice, Inbred C57BL
  • Mice
  • Melanoma-Specific Antigens
  • Liver Neoplasms
  • Liver Cirrhosis
  • Humans
 

Citation

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ICMJE
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Kannangai, R., Diehl, A. M., Sicklick, J., Rojkind, M., Thomas, D., & Torbenson, M. (2005). Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile. Hum Pathol, 36(4), 341–347. https://doi.org/10.1016/j.humpath.2005.01.002
Kannangai, Rajesh, Anna Mae Diehl, Jason Sicklick, Marcus Rojkind, David Thomas, and Michael Torbenson. “Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile.Hum Pathol 36, no. 4 (April 2005): 341–47. https://doi.org/10.1016/j.humpath.2005.01.002.
Kannangai R, Diehl AM, Sicklick J, Rojkind M, Thomas D, Torbenson M. Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile. Hum Pathol. 2005 Apr;36(4):341–7.
Kannangai, Rajesh, et al. “Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile.Hum Pathol, vol. 36, no. 4, Apr. 2005, pp. 341–47. Pubmed, doi:10.1016/j.humpath.2005.01.002.
Kannangai R, Diehl AM, Sicklick J, Rojkind M, Thomas D, Torbenson M. Hepatic angiomyolipoma and hepatic stellate cells share a similar gene expression profile. Hum Pathol. 2005 Apr;36(4):341–347.
Journal cover image

Published In

Hum Pathol

DOI

ISSN

0046-8177

Publication Date

April 2005

Volume

36

Issue

4

Start / End Page

341 / 347

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Pathology
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Mice, Inbred C57BL
  • Mice
  • Melanoma-Specific Antigens
  • Liver Neoplasms
  • Liver Cirrhosis
  • Humans