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Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus).

Publication ,  Journal Article
Stajich, JE; Wilke, SK; Ahrén, D; Au, CH; Birren, BW; Borodovsky, M; Burns, C; Canbäck, B; Casselton, LA; Cheng, CK; Deng, J; Dietrich, FS ...
Published in: Proc Natl Acad Sci U S A
June 29, 2010

The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle in 2 weeks, produces some 10(8) synchronized meiocytes, and can be manipulated at all stages in development by mutation and transformation. The 37-megabase genome of C. cinerea was sequenced and assembled into 13 chromosomes. Meiotic recombination rates vary greatly along the chromosomes, and retrotransposons are absent in large regions of the genome with low levels of meiotic recombination. Single-copy genes with identifiable orthologs in other basidiomycetes are predominant in low-recombination regions of the chromosome. In contrast, paralogous multicopy genes are found in the highly recombining regions, including a large family of protein kinases (FunK1) unique to multicellular fungi. Analyses of P450 and hydrophobin gene families confirmed that local gene duplications drive the expansions of paralogous copies and the expansions occur in independent lineages of Agaricomycotina fungi. Gene-expression patterns from microarrays were used to dissect the transcriptional program of dikaryon formation (mating). Several members of the FunK1 kinase family are differentially regulated during sexual morphogenesis, and coordinate regulation of adjacent duplications is rare. The genomes of C. cinerea and Laccaria bicolor, a symbiotic basidiomycete, share extensive regions of synteny. The largest syntenic blocks occur in regions with low meiotic recombination rates, no transposable elements, and tight gene spacing, where orthologous single-copy genes are overrepresented. The chromosome assembly of C. cinerea is an essential resource in understanding the evolution of multicellularity in the fungi.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

June 29, 2010

Volume

107

Issue

26

Start / End Page

11889 / 11894

Location

United States

Related Subject Headings

  • Retroelements
  • Recombination, Genetic
  • RNA, Fungal
  • Protein Kinases
  • Phylogeny
  • Multigene Family
  • Molecular Sequence Data
  • Meiosis
  • Genome, Fungal
  • Gene Duplication
 

Citation

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Stajich, J. E., Wilke, S. K., Ahrén, D., Au, C. H., Birren, B. W., Borodovsky, M., … Pukkila, P. J. (2010). Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proc Natl Acad Sci U S A, 107(26), 11889–11894. https://doi.org/10.1073/pnas.1003391107
Stajich, Jason E., Sarah K. Wilke, Dag Ahrén, Chun Hang Au, Bruce W. Birren, Mark Borodovsky, Claire Burns, et al. “Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus).Proc Natl Acad Sci U S A 107, no. 26 (June 29, 2010): 11889–94. https://doi.org/10.1073/pnas.1003391107.
Stajich JE, Wilke SK, Ahrén D, Au CH, Birren BW, Borodovsky M, et al. Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11889–94.
Stajich, Jason E., et al. “Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus).Proc Natl Acad Sci U S A, vol. 107, no. 26, June 2010, pp. 11889–94. Pubmed, doi:10.1073/pnas.1003391107.
Stajich JE, Wilke SK, Ahrén D, Au CH, Birren BW, Borodovsky M, Burns C, Canbäck B, Casselton LA, Cheng CK, Deng J, Dietrich FS, Fargo DC, Farman ML, Gathman AC, Goldberg J, Guigó R, Hoegger PJ, Hooker JB, Huggins A, James TY, Kamada T, Kilaru S, Kodira C, Kües U, Kupfer D, Kwan HS, Lomsadze A, Li W, Lilly WW, Ma L-J, Mackey AJ, Manning G, Martin F, Muraguchi H, Natvig DO, Palmerini H, Ramesh MA, Rehmeyer CJ, Roe BA, Shenoy N, Stanke M, Ter-Hovhannisyan V, Tunlid A, Velagapudi R, Vision TJ, Zeng Q, Zolan ME, Pukkila PJ. Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11889–11894.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

June 29, 2010

Volume

107

Issue

26

Start / End Page

11889 / 11894

Location

United States

Related Subject Headings

  • Retroelements
  • Recombination, Genetic
  • RNA, Fungal
  • Protein Kinases
  • Phylogeny
  • Multigene Family
  • Molecular Sequence Data
  • Meiosis
  • Genome, Fungal
  • Gene Duplication