Scholars@Duke publication: The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer.

The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer.

Publication , Journal Article

Nakajima, M; Hutchinson, HG; Fujinaga, M; Hayashida, W; Morishita, R; Zhang, L; Horiuchi, M; Pratt, RE; Dzau, VJ

Published in: Proc Natl Acad Sci U S A

November 7, 1995

The type 1 angiotensin II (AT1) receptor is well characterized but the type 2 (AT2) receptor remains an enigma. We tested the hypothesis that the AT2 receptor can modulate the growth of vascular smooth muscle cells by transfecting an AT2 receptor expression vector into the balloon-injured rat carotid artery and observed that overexpression of the AT2 receptor attenuated neointimal formation. In cultured smooth muscle cells, AT2 receptor transfection reduced proliferation and inhibited mitogen-activated protein kinase activity. Furthermore, we demonstrated that the AT2 receptor mediated the developmentally regulated decrease in aortic DNA synthesis at the latter stages of gestation. These results suggest that the AT2 receptor exerts an antiproliferative effect, counteracting the growth action of AT1 receptor.

Duke Scholars

Author Victor J. Dzau Medicine, Cardiology

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.92.23.10663

ISSN

0027-8424

Publication Date

November 7, 1995

Volume

Issue

Start / End Page

10663 / 10667

Location

United States

Related Subject Headings

Tunica Intima
Transfection
Receptors, Angiotensin
Rats
Radioligand Assay
Polymerase Chain Reaction
Muscle, Smooth, Vascular
Muscle Development
Molecular Sequence Data
Cells, Cultured

Citation

APA

Chicago

ICMJE

MLA

NLM

Nakajima, M., Hutchinson, H. G., Fujinaga, M., Hayashida, W., Morishita, R., Zhang, L., … Dzau, V. J. (1995). The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer. Proc Natl Acad Sci U S A, 92(23), 10663–10667. https://doi.org/10.1073/pnas.92.23.10663

Nakajima, M., H. G. Hutchinson, M. Fujinaga, W. Hayashida, R. Morishita, L. Zhang, M. Horiuchi, R. E. Pratt, and V. J. Dzau. “The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer.” Proc Natl Acad Sci U S A 92, no. 23 (November 7, 1995): 10663–67. https://doi.org/10.1073/pnas.92.23.10663.

Nakajima M, Hutchinson HG, Fujinaga M, Hayashida W, Morishita R, Zhang L, et al. The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer. Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10663–7.

Nakajima, M., et al. “The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer.” Proc Natl Acad Sci U S A, vol. 92, no. 23, Nov. 1995, pp. 10663–67. Pubmed, doi:10.1073/pnas.92.23.10663.

Nakajima M, Hutchinson HG, Fujinaga M, Hayashida W, Morishita R, Zhang L, Horiuchi M, Pratt RE, Dzau VJ. The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer. Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10663–10667.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.92.23.10663

ISSN

0027-8424

Publication Date

November 7, 1995

Volume

Issue

Start / End Page

10663 / 10667

Location

United States

Related Subject Headings

Tunica Intima
Transfection
Receptors, Angiotensin
Rats
Radioligand Assay
Polymerase Chain Reaction
Muscle, Smooth, Vascular
Muscle Development
Molecular Sequence Data
Cells, Cultured