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Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II.

Publication ,  Journal Article
Itoh, H; Mukoyama, M; Pratt, RE; Gibbons, GH; Dzau, VJ
Published in: J Clin Invest
May 1993

Angiotensin (Ang) II stimulates hypertrophic growth of vascular smooth muscle cells (VSMC). Accompanying this growth is the induction of the expression of growth-related protooncogenes (c-fos, c-jun, and c-myc), as well as the synthesis of the autocrine growth factors, such as PDGF-A and TGF-beta 1. In this study, we demonstrate further that Ang II also induces the synthesis of basic fibroblast growth factor (bFGF), a potent mitogen for VSMC. To examine how these factors interact to modulate the growth response of VSMC to Ang II, we used antisense oligomers to determine the relative contribution of these three factors. Treatment of confluent, quiescent smooth muscle cells with specific antisense oligomers complementary to bFGF, PDGF-A, and TGF-beta 1 efficiently inhibited the syntheses of these factors. Our results demonstrate that in these VSMC, TGF-beta 1 affects a key antiproliferative action, modulating the mitogenic properties of bFGF. Autocrine PDGF exerts only a minimal effect on DNA synthesis. An imbalance in these signals activated by Ang II may result in abnormal VSMC growth leading to the development of vascular disease.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 1993

Volume

91

Issue

5

Start / End Page

2268 / 2274

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Rats
  • Platelet-Derived Growth Factor
  • Oligonucleotides, Antisense
  • Oligodeoxyribonucleotides
  • Muscle, Smooth, Vascular
  • Molecular Sequence Data
  • Mice
  • Kinetics
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Itoh, H., Mukoyama, M., Pratt, R. E., Gibbons, G. H., & Dzau, V. J. (1993). Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II. J Clin Invest, 91(5), 2268–2274. https://doi.org/10.1172/JCI116454
Itoh, H., M. Mukoyama, R. E. Pratt, G. H. Gibbons, and V. J. Dzau. “Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II.J Clin Invest 91, no. 5 (May 1993): 2268–74. https://doi.org/10.1172/JCI116454.
Itoh H, Mukoyama M, Pratt RE, Gibbons GH, Dzau VJ. Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II. J Clin Invest. 1993 May;91(5):2268–74.
Itoh, H., et al. “Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II.J Clin Invest, vol. 91, no. 5, May 1993, pp. 2268–74. Pubmed, doi:10.1172/JCI116454.
Itoh H, Mukoyama M, Pratt RE, Gibbons GH, Dzau VJ. Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II. J Clin Invest. 1993 May;91(5):2268–2274.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 1993

Volume

91

Issue

5

Start / End Page

2268 / 2274

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Rats
  • Platelet-Derived Growth Factor
  • Oligonucleotides, Antisense
  • Oligodeoxyribonucleotides
  • Muscle, Smooth, Vascular
  • Molecular Sequence Data
  • Mice
  • Kinetics
  • Immunology