Electron microscopic study of base sequence in nucleic acids VI. Guanine sites in yeast alanine transfer RNA

Yeast alanine t-RNA was reacted with 2-diazo-p-benzene-disulphonic acid, which is known to attach with high specificity to the guanosine monophosphate nucleotides. The guanine marked RNA molecules were extended on thin carbon film, stained with uranyl acetate, and examined in the electron microscope. In high resolution micrographs most of the markers made visible by the uranyl acetate staining, could be distinguished from the background noise on the basis of contrast and size. They were found in linear arrays with a length not exceeding that of an extended t-RNA molecule and their number not exceeding that expected from chemical reactivity data as well as from the known number of guanosine residues in ala t-RNA molecule. © 1970.

Duke Scholars

Author Harold Paul Erickson Cell Biology