Skip to main content
Journal cover image

Structural features of nephritogenic lupus autoantibodies.

Publication ,  Journal Article
Vargas, MT; Gustilo, K; D'Andrea, DM; Kalluri, R; Foster, MH; Madaio, MP
Published in: Methods
January 1997

We have identified monoclonal antibodies derived from MRL-lpr/lpr lupus-prone mice that produced nephritis after passive transfer to normal mice. Our present goal was to elucidate the structural and immunochemical features of nephritogenic Ig that facilitate immune deposition. For this purpose the antigen binding properties, capacity to form immune deposits, and nucleotide sequence of a genetically related autoantibody subgroup were compared. The prototype, H147 (an IgG encoded by 7183/81X VH gene), produced glomerular and tubular basement membrane, mesangial immune deposits, and proliferative glomerulonephritis after passive transfer to normal mice. For comparison three other 7183/81X encoded anti-DNA IgG (H257, H171, and H8a) were evaluated (predicted heavy chain aa homology >75%). H257 produced similar types of immune deposits as H147, and this was associated with nephritis; H8a produced predominantly mesangial deposits, whereas H171 did not produce significant deposits. Although their antigen binding profile to a panel of soluble autoantigens was variable, only H147 and H257 bound to both mesangial and aortic endothelial cell surfaces. V gene sequence analysis of the IgG suggests that individual residues, motifs, and conformations influence the autoantigen binding specificities that contributed to the observed differences in immune deposit formation.

Duke Scholars

Published In

Methods

DOI

ISSN

1046-2023

Publication Date

January 1997

Volume

11

Issue

1

Start / End Page

62 / 69

Location

United States

Related Subject Headings

  • Sequence Analysis
  • Molecular Sequence Data
  • Mice, Inbred Strains
  • Mice
  • Lupus Nephritis
  • Kidney
  • Immunoglobulin Variable Region
  • Hybridomas
  • Glomerular Mesangium
  • Cloning, Molecular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vargas, M. T., Gustilo, K., D’Andrea, D. M., Kalluri, R., Foster, M. H., & Madaio, M. P. (1997). Structural features of nephritogenic lupus autoantibodies. Methods, 11(1), 62–69. https://doi.org/10.1006/meth.1996.0388
Vargas, M. T., K. Gustilo, D. M. D’Andrea, R. Kalluri, M. H. Foster, and M. P. Madaio. “Structural features of nephritogenic lupus autoantibodies.Methods 11, no. 1 (January 1997): 62–69. https://doi.org/10.1006/meth.1996.0388.
Vargas MT, Gustilo K, D’Andrea DM, Kalluri R, Foster MH, Madaio MP. Structural features of nephritogenic lupus autoantibodies. Methods. 1997 Jan;11(1):62–9.
Vargas, M. T., et al. “Structural features of nephritogenic lupus autoantibodies.Methods, vol. 11, no. 1, Jan. 1997, pp. 62–69. Pubmed, doi:10.1006/meth.1996.0388.
Vargas MT, Gustilo K, D’Andrea DM, Kalluri R, Foster MH, Madaio MP. Structural features of nephritogenic lupus autoantibodies. Methods. 1997 Jan;11(1):62–69.
Journal cover image

Published In

Methods

DOI

ISSN

1046-2023

Publication Date

January 1997

Volume

11

Issue

1

Start / End Page

62 / 69

Location

United States

Related Subject Headings

  • Sequence Analysis
  • Molecular Sequence Data
  • Mice, Inbred Strains
  • Mice
  • Lupus Nephritis
  • Kidney
  • Immunoglobulin Variable Region
  • Hybridomas
  • Glomerular Mesangium
  • Cloning, Molecular