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Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium.

Publication ,  Journal Article
Guan, Z; Wang, X; Raetz, CRH
Published in: Anal Biochem
February 15, 2011

Two homologous 29 amino acid-long highly hydrophobic membrane miniproteins were identified in the Bligh-Dyer lipid extracts of Escherichia coli and Salmonella typhimurium using liquid chromatography/tandem mass spectrometry (LC/MS/MS). The amino acid sequences of the proteins were determined by collision-induced dissociation tandem mass spectrometry, in conjunction with a translating BLAST (tBLASTn) search, i.e., comparing the MS/MS-determined protein query sequence against the six-frame translations of the nucleotide sequences of the E. coli and S. typhimurium genomes. Further MS characterization revealed that both proteins retain the N-terminal initiating formyl-methionines. The methodologies described here may be amendable for detecting and characterizing small hydrophobic proteins in other organisms that are difficult to annotate or analyze by conventional methods.

Duke Scholars

Published In

Anal Biochem

DOI

EISSN

1096-0309

Publication Date

February 15, 2011

Volume

409

Issue

2

Start / End Page

284 / 289

Location

United States

Related Subject Headings

  • Spectrometry, Mass, Electrospray Ionization
  • Salmonella typhimurium
  • Molecular Sequence Data
  • Membrane Proteins
  • Mass Spectrometry
  • Genome, Bacterial
  • Escherichia coli Proteins
  • Escherichia coli
  • Chromatography, Liquid
  • Chloroform
 

Citation

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Guan, Z., Wang, X., & Raetz, C. R. H. (2011). Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium. Anal Biochem, 409(2), 284–289. https://doi.org/10.1016/j.ab.2010.10.035
Guan, Ziqiang, Xiaoyuan Wang, and Christian R. H. Raetz. “Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium.Anal Biochem 409, no. 2 (February 15, 2011): 284–89. https://doi.org/10.1016/j.ab.2010.10.035.
Guan, Ziqiang, et al. “Identification of a chloroform-soluble membrane miniprotein in Escherichia coli and its homolog in Salmonella typhimurium.Anal Biochem, vol. 409, no. 2, Feb. 2011, pp. 284–89. Pubmed, doi:10.1016/j.ab.2010.10.035.
Journal cover image

Published In

Anal Biochem

DOI

EISSN

1096-0309

Publication Date

February 15, 2011

Volume

409

Issue

2

Start / End Page

284 / 289

Location

United States

Related Subject Headings

  • Spectrometry, Mass, Electrospray Ionization
  • Salmonella typhimurium
  • Molecular Sequence Data
  • Membrane Proteins
  • Mass Spectrometry
  • Genome, Bacterial
  • Escherichia coli Proteins
  • Escherichia coli
  • Chromatography, Liquid
  • Chloroform