Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.
Publication
, Journal Article
Guan, Z; Breazeale, SD; Raetz, CRH
Published in: Anal Biochem
October 15, 2005
Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.
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Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 15, 2005
Volume
345
Issue
2
Start / End Page
336 / 339
Location
United States
Related Subject Headings
- Spectrometry, Mass, Electrospray Ionization
- Solubility
- Muramic Acids
- Mass Spectrometry
- Hydrogen-Ion Concentration
- Escherichia coli
- Diphosphates
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
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Chicago
ICMJE
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Guan, Z., Breazeale, S. D., & Raetz, C. R. H. (2005). Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli. Anal Biochem, 345(2), 336–339. https://doi.org/10.1016/j.ab.2005.07.002
Guan, Ziqiang, Steven D. Breazeale, and Christian R. H. Raetz. “Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.” Anal Biochem 345, no. 2 (October 15, 2005): 336–39. https://doi.org/10.1016/j.ab.2005.07.002.
Guan Z, Breazeale SD, Raetz CRH. Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli. Anal Biochem. 2005 Oct 15;345(2):336–9.
Guan, Ziqiang, et al. “Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.” Anal Biochem, vol. 345, no. 2, Oct. 2005, pp. 336–39. Pubmed, doi:10.1016/j.ab.2005.07.002.
Guan Z, Breazeale SD, Raetz CRH. Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli. Anal Biochem. 2005 Oct 15;345(2):336–339.
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 15, 2005
Volume
345
Issue
2
Start / End Page
336 / 339
Location
United States
Related Subject Headings
- Spectrometry, Mass, Electrospray Ionization
- Solubility
- Muramic Acids
- Mass Spectrometry
- Hydrogen-Ion Concentration
- Escherichia coli
- Diphosphates
- Biochemistry & Molecular Biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences