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Isolation and insecticidal activity of mellamide from Aspergillus melleus.

Publication ,  Journal Article
Ondeyka, JG; Dombrowski, AW; Polishook, JP; Felcetto, T; Shoop, WL; Guan, Z; Singh, SB
Published in: J Ind Microbiol Biotechnol
April 2003

Mellamide, a novel indole amide, was isolated from a fermentation of Aspergillus melleus using silica gel and high-performance liquid chromatographic methods. This allowed its separation from three known antiparasitic compounds (ochratoxin A, viomellin and xanthomegnin) also present in the potent extract. The structure was elucidated by (1)H, (13)C, COSY, DEPT, HMQC and HMBC NMR experiments. HR-FTMS aided in the molecular weight and formula determination. Mellamide showed in vitro insecticidal activity in bioassays against larvae of Lucilia sericata and Aedes egypti with LD(90) of 1,000 and 50 micro g/ml, respectively.

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Published In

J Ind Microbiol Biotechnol

DOI

ISSN

1367-5435

Publication Date

April 2003

Volume

30

Issue

4

Start / End Page

220 / 224

Location

Germany

Related Subject Headings

  • Larva
  • Insecticides
  • Industrial Microbiology
  • Indoles
  • Diptera
  • Culicidae
  • Biotechnology
  • Biological Assay
  • Aspergillus
  • Animals
 

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Ondeyka, J. G., Dombrowski, A. W., Polishook, J. P., Felcetto, T., Shoop, W. L., Guan, Z., & Singh, S. B. (2003). Isolation and insecticidal activity of mellamide from Aspergillus melleus. J Ind Microbiol Biotechnol, 30(4), 220–224. https://doi.org/10.1007/s10295-003-0048-y
Ondeyka, J. G., A. W. Dombrowski, J. P. Polishook, T. Felcetto, W. L. Shoop, Z. Guan, and S. B. Singh. “Isolation and insecticidal activity of mellamide from Aspergillus melleus.J Ind Microbiol Biotechnol 30, no. 4 (April 2003): 220–24. https://doi.org/10.1007/s10295-003-0048-y.
Ondeyka JG, Dombrowski AW, Polishook JP, Felcetto T, Shoop WL, Guan Z, et al. Isolation and insecticidal activity of mellamide from Aspergillus melleus. J Ind Microbiol Biotechnol. 2003 Apr;30(4):220–4.
Ondeyka, J. G., et al. “Isolation and insecticidal activity of mellamide from Aspergillus melleus.J Ind Microbiol Biotechnol, vol. 30, no. 4, Apr. 2003, pp. 220–24. Pubmed, doi:10.1007/s10295-003-0048-y.
Ondeyka JG, Dombrowski AW, Polishook JP, Felcetto T, Shoop WL, Guan Z, Singh SB. Isolation and insecticidal activity of mellamide from Aspergillus melleus. J Ind Microbiol Biotechnol. 2003 Apr;30(4):220–224.
Journal cover image

Published In

J Ind Microbiol Biotechnol

DOI

ISSN

1367-5435

Publication Date

April 2003

Volume

30

Issue

4

Start / End Page

220 / 224

Location

Germany

Related Subject Headings

  • Larva
  • Insecticides
  • Industrial Microbiology
  • Indoles
  • Diptera
  • Culicidae
  • Biotechnology
  • Biological Assay
  • Aspergillus
  • Animals