Double stranded DNA sequencing by tandem mass spectrometry
Publication
, Journal Article
McLafferty, FW; Aaserud, DJ; Guan, Z; Little, DP; Kelleher, NL
Published in: International Journal of Mass Spectrometry and Ion Processes
January 1, 1997
Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3′-end heterogeneity. Published by Elsevier Science B.V.
Duke Scholars
Published In
International Journal of Mass Spectrometry and Ion Processes
DOI
ISSN
0168-1176
Publication Date
January 1, 1997
Volume
165-166
Start / End Page
457 / 466
Related Subject Headings
- Analytical Chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
McLafferty, F. W., Aaserud, D. J., Guan, Z., Little, D. P., & Kelleher, N. L. (1997). Double stranded DNA sequencing by tandem mass spectrometry. International Journal of Mass Spectrometry and Ion Processes, 165–166, 457–466. https://doi.org/10.1016/s0168-1176(97)00171-7
McLafferty, F. W., D. J. Aaserud, Z. Guan, D. P. Little, and N. L. Kelleher. “Double stranded DNA sequencing by tandem mass spectrometry.” International Journal of Mass Spectrometry and Ion Processes 165–166 (January 1, 1997): 457–66. https://doi.org/10.1016/s0168-1176(97)00171-7.
McLafferty FW, Aaserud DJ, Guan Z, Little DP, Kelleher NL. Double stranded DNA sequencing by tandem mass spectrometry. International Journal of Mass Spectrometry and Ion Processes. 1997 Jan 1;165–166:457–66.
McLafferty, F. W., et al. “Double stranded DNA sequencing by tandem mass spectrometry.” International Journal of Mass Spectrometry and Ion Processes, vol. 165–166, Jan. 1997, pp. 457–66. Scopus, doi:10.1016/s0168-1176(97)00171-7.
McLafferty FW, Aaserud DJ, Guan Z, Little DP, Kelleher NL. Double stranded DNA sequencing by tandem mass spectrometry. International Journal of Mass Spectrometry and Ion Processes. 1997 Jan 1;165–166:457–466.
Published In
International Journal of Mass Spectrometry and Ion Processes
DOI
ISSN
0168-1176
Publication Date
January 1, 1997
Volume
165-166
Start / End Page
457 / 466
Related Subject Headings
- Analytical Chemistry