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Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines.

Publication ,  Journal Article
Bhattacharya, SD; Garrison, J; Guo, H; Mi, Z; Markovic, J; Kim, VM; Kuo, PC
Published in: Surgery
August 2010

BACKGROUND: Osteopontin (OPN) is a variably expressed, secreted glycophosphoprotein that mediates the growth and metastases of hepatocellular cancer (HCC). MicroRNAs (miRNAs) may be responsible for variant OPN expression, interrupting translation by binding OPN messenger RNA (mRNA) in 3'-untranslated regions (UTRs). METHODS: A microarray analysis identified miRNAs of interest. Plasmid constructs using a luciferase reporter with variable OPN 3'UTR mutations were transfected into 2 HCC cell lines to determine miRNA regulation of OPN expression. Western blot analyses confirmed variable OPN expression in both cell lines. Invasion, adhesion, and migration evaluated metastatic behavior in Hep G2 and Hep 3B with modified miRNA and OPN expression. RESULTS: Hep 3B produces 36 x miRNA 181a compared with Hep G2. Luciferase activity after transfection with miRNA 181a precursor was decreased in both cell lines (P < .01); luciferase activity increased with miRNA 181a inhibitor transfection in both cell lines (P < .01). Hep 3B transfected with mutated OPN 3'UTR increased luciferase activity 108% (P < .01). Hep G2 transfected with miRNA precursor decreased OPN expression 5 x (P < .01) in Western blot analyses. Hep 3B transfection with miRNA precursor increased OPN expression 3 x (P < .01) in Western blot analyses. In vitro metastatic correlates increased in Hep 3B lines after transfection with siOPN and/or miRNA 181a inhibitor (P < .01). CONCLUSION: MiRNA 181a decreases OPN expression in HCC cell lines. This previously undescribed mechanism may confer metastatic characteristics to HCC.

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Published In

Surgery

DOI

EISSN

1532-7361

Publication Date

August 2010

Volume

148

Issue

2

Start / End Page

291 / 297

Location

United States

Related Subject Headings

  • Transfection
  • Surgery
  • RNA, Small Interfering
  • RNA, Neoplasm
  • Osteopontin
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Invasiveness
  • Mutation
  • Mutagenesis, Site-Directed
  • MicroRNAs
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bhattacharya, S. D., Garrison, J., Guo, H., Mi, Z., Markovic, J., Kim, V. M., & Kuo, P. C. (2010). Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines. Surgery, 148(2), 291–297. https://doi.org/10.1016/j.surg.2010.05.007
Bhattacharya, Syamal D., Juline Garrison, Hongtao Guo, Zhiyong Mi, Jovan Markovic, Victoria M. Kim, and Paul C. Kuo. “Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines.Surgery 148, no. 2 (August 2010): 291–97. https://doi.org/10.1016/j.surg.2010.05.007.
Bhattacharya SD, Garrison J, Guo H, Mi Z, Markovic J, Kim VM, et al. Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines. Surgery. 2010 Aug;148(2):291–7.
Bhattacharya, Syamal D., et al. “Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines.Surgery, vol. 148, no. 2, Aug. 2010, pp. 291–97. Pubmed, doi:10.1016/j.surg.2010.05.007.
Bhattacharya SD, Garrison J, Guo H, Mi Z, Markovic J, Kim VM, Kuo PC. Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines. Surgery. 2010 Aug;148(2):291–297.
Journal cover image

Published In

Surgery

DOI

EISSN

1532-7361

Publication Date

August 2010

Volume

148

Issue

2

Start / End Page

291 / 297

Location

United States

Related Subject Headings

  • Transfection
  • Surgery
  • RNA, Small Interfering
  • RNA, Neoplasm
  • Osteopontin
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Invasiveness
  • Mutation
  • Mutagenesis, Site-Directed
  • MicroRNAs