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Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight

Publication ,  Journal Article
Johanson, K; L. Allen, P; Gonzalez-Villalobos, RA; Nesbit, J; Nickerson, CA; Höner zu Bentrup, K; Wilson, JW; Ramamurthy, R; D'Elia, R; Muse, KE ...
Published in: Acta Astronautica
February 1, 2007

This study identifies genes that determine survival during a space flight, using the model eukaryotic organism, Saccharomyces cerevisiae. Select strains of a haploid yeast deletion series grew during storage in distilled water in space, but not in ground based static or clinorotation controls. The survival advantages in space in distilled water include a 133-fold advantage for the deletion of PEX19, a chaperone and import receptor for newly- synthesized class I peroxisomal membrane proteins, to 77-40 fold for deletion strains lacking elements of aerobic respiration, isocitrate metabolism, and mitochondrial electron transport. Following automated addition of rich growth media, the space flight was associated with a marked survival advantage of strains with deletions in catalytically active genes including hydrolases, oxidoreductases and transferases. When compared to static controls, space flight was associated with a marked survival disadvantage of deletion strains lacking transporter, antioxidant and catalytic activity. This study identifies yeast deletion strains with a survival advantage during storage in distilled water and space flight, and amplifies our understanding of the genes critical for survival in space.

Duke Scholars

Published In

Acta Astronautica

DOI

ISSN

0094-5765

Publication Date

February 1, 2007

Volume

60

Issue

4-7 SPEC. ISS.

Start / End Page

460 / 471

Related Subject Headings

  • Aerospace & Aeronautics
  • 0913 Mechanical Engineering
  • 0901 Aerospace Engineering
 

Citation

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Johanson, K., L. Allen, P., Gonzalez-Villalobos, R. A., Nesbit, J., Nickerson, C. A., Höner zu Bentrup, K., … Hammond, T. G. (2007). Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. Acta Astronautica, 60(4-7 SPEC. ISS.), 460–471. https://doi.org/10.1016/j.actaastro.2006.09.011
Johanson, K., P. L. Allen, R. A. Gonzalez-Villalobos, J. Nesbit, C. A. Nickerson, K. Höner zu Bentrup, J. W. Wilson, et al. “Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight.” Acta Astronautica 60, no. 4-7 SPEC. ISS. (February 1, 2007): 460–71. https://doi.org/10.1016/j.actaastro.2006.09.011.
Johanson K, L. Allen P, Gonzalez-Villalobos RA, Nesbit J, Nickerson CA, Höner zu Bentrup K, et al. Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. Acta Astronautica. 2007 Feb 1;60(4-7 SPEC. ISS.):460–71.
Johanson, K., et al. “Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight.” Acta Astronautica, vol. 60, no. 4-7 SPEC. ISS., Feb. 2007, pp. 460–71. Scopus, doi:10.1016/j.actaastro.2006.09.011.
Johanson K, L. Allen P, Gonzalez-Villalobos RA, Nesbit J, Nickerson CA, Höner zu Bentrup K, Wilson JW, Ramamurthy R, D’Elia R, Muse KE, Hammond J, Freeman J, Stodieck LS, Hammond TG. Haploid deletion strains of Saccharomyces cerevisiae that determine survival during space flight. Acta Astronautica. 2007 Feb 1;60(4-7 SPEC. ISS.):460–471.
Journal cover image

Published In

Acta Astronautica

DOI

ISSN

0094-5765

Publication Date

February 1, 2007

Volume

60

Issue

4-7 SPEC. ISS.

Start / End Page

460 / 471

Related Subject Headings

  • Aerospace & Aeronautics
  • 0913 Mechanical Engineering
  • 0901 Aerospace Engineering