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Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism.

Publication ,  Journal Article
Lebel-Hardenack, S; Hauser, E; Law, TF; Schmid, J; Grant, SR
Published in: Genetics
February 2002

S. latifolia is a dioecious plant with morphologically distinct sex chromosomes. To genetically map the sex determination loci on the male-specific Y chromosome, we identified X-ray-induced sex determination mutants that had lost male traits. We used male-specific AFLP markers to characterize the extent of deletions in the Y chromosomes of the mutants. We then compared overlapping deletions to predict the order of the AFLP markers and to locate the mutated sex-determining genes. We found three regions on the Y chromosome where frequent deletions were significantly associated with loss of male traits. One was associated with hermaphroditic mutants. A second was associated with asexual mutants that lack genes needed for early stamen development and a third was associated with asexual mutants that lack genes for late stages of stamen development. Our observations confirmed a classical genetic prediction that S. latifolia has three dispersed male-determining loci on the Y chromosome, one for carpel suppression, one for early stamen development, and another for late stamen development. This AFLP map provides a framework for locating genes on the Y chromosome and for characterizing deletions on the Y chromosomes of potentially interesting mutants.

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Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

February 2002

Volume

160

Issue

2

Start / End Page

717 / 725

Location

United States

Related Subject Headings

  • Y Chromosome
  • Silene
  • Sex Determination Processes
  • Nucleic Acid Amplification Techniques
  • Mutation
  • Genetic Markers
  • Gene Deletion
  • Developmental Biology
  • Crosses, Genetic
  • Chromosome Mapping
 

Citation

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ICMJE
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Lebel-Hardenack, S., Hauser, E., Law, T. F., Schmid, J., & Grant, S. R. (2002). Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism. Genetics, 160(2), 717–725. https://doi.org/10.1093/genetics/160.2.717
Lebel-Hardenack, Sabine, Elizabeth Hauser, Teresa F. Law, Jurg Schmid, and Sarah R. Grant. “Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism.Genetics 160, no. 2 (February 2002): 717–25. https://doi.org/10.1093/genetics/160.2.717.
Lebel-Hardenack S, Hauser E, Law TF, Schmid J, Grant SR. Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism. Genetics. 2002 Feb;160(2):717–25.
Lebel-Hardenack, Sabine, et al. “Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism.Genetics, vol. 160, no. 2, Feb. 2002, pp. 717–25. Pubmed, doi:10.1093/genetics/160.2.717.
Lebel-Hardenack S, Hauser E, Law TF, Schmid J, Grant SR. Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism. Genetics. 2002 Feb;160(2):717–725.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

February 2002

Volume

160

Issue

2

Start / End Page

717 / 725

Location

United States

Related Subject Headings

  • Y Chromosome
  • Silene
  • Sex Determination Processes
  • Nucleic Acid Amplification Techniques
  • Mutation
  • Genetic Markers
  • Gene Deletion
  • Developmental Biology
  • Crosses, Genetic
  • Chromosome Mapping