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The colorectal microRNAome.

Publication ,  Journal Article
Cummins, JM; He, Y; Leary, RJ; Pagliarini, R; Diaz, LA; Sjoblom, T; Barad, O; Bentwich, Z; Szafranska, AE; Labourier, E; Raymond, CK; Juhl, H ...
Published in: Proc Natl Acad Sci U S A
March 7, 2006
Published version (DOI) Link to item

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues.

Duke Scholars

Yiping He
Author Yiping He Pathology

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.0511155103

ISSN

0027-8424

Publication Date

March 7, 2006

Volume

103

Issue

10

Start / End Page

3687 / 3692

Location

United States

Related Subject Headings

  • Ribonuclease III
  • Rectum
  • RNA, Neoplasm
  • RNA Helicases
  • Nucleic Acid Conformation
  • MicroRNAs
  • Humans
  • Genome, Human
  • Gene Targeting
  • Gene Expression
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cummins, J. M., He, Y., Leary, R. J., Pagliarini, R., Diaz, L. A., Sjoblom, T., … Velculescu, V. E. (2006). The colorectal microRNAome. Proc Natl Acad Sci U S A, 103(10), 3687–3692. https://doi.org/10.1073/pnas.0511155103
Cummins, Jordan M., Yiping He, Rebecca J. Leary, Ray Pagliarini, Luis A. Diaz, Tobias Sjoblom, Omer Barad, et al. “The colorectal microRNAome.” Proc Natl Acad Sci U S A 103, no. 10 (March 7, 2006): 3687–92. https://doi.org/10.1073/pnas.0511155103.
Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA, Sjoblom T, et al. The colorectal microRNAome. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3687–92.
Cummins, Jordan M., et al. “The colorectal microRNAome.” Proc Natl Acad Sci U S A, vol. 103, no. 10, Mar. 2006, pp. 3687–92. Pubmed, doi:10.1073/pnas.0511155103.
Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, Juhl H, Kinzler KW, Vogelstein B, Velculescu VE. The colorectal microRNAome. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3687–3692.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.0511155103

ISSN

0027-8424

Publication Date

March 7, 2006

Volume

103

Issue

10

Start / End Page

3687 / 3692

Location

United States

Related Subject Headings

  • Ribonuclease III
  • Rectum
  • RNA, Neoplasm
  • RNA Helicases
  • Nucleic Acid Conformation
  • MicroRNAs
  • Humans
  • Genome, Human
  • Gene Targeting
  • Gene Expression