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Culture of endodermal stem/progenitor cells of the mouse tongue.

Publication ,  Journal Article
Luo, X; Okubo, T; Randell, S; Hogan, BLM
Published in: In Vitro Cell Dev Biol Anim
2009

The tongue represents a very accessible source of tissue-specific epithelial stem cells of endodermal origin. However, little is known about the properties of these cells and the mechanisms regulating their proliferation and differentiation. Foxa2, an endodermal marker, is expressed throughout the tongue epithelium during embryonic development but becomes confined to a minority of basal cells and some taste bud sensory cells in the adult tongue. Using a previously described line of transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of a human keratin 5 promoter region (Krt5-eGFP), we have isolated a subpopulation of cells in the basal epithelial layer of the mouse tongue with a high efficiency of generating holoclones of undifferentiated cells in culture with a feeder layer. Krt5-GFP(hi) cells can both self renew and give rise to differentiated stratified keratinized epithelial cells when cultured on an air-liquid interface.

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Published In

In Vitro Cell Dev Biol Anim

DOI

EISSN

1543-706X

Publication Date

2009

Volume

45

Issue

1-2

Start / End Page

44 / 54

Location

Germany

Related Subject Headings

  • Tongue
  • Taste Buds
  • Stem Cells
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Humans
  • Female
  • Epithelial Cells
 

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Luo, X., Okubo, T., Randell, S., & Hogan, B. L. M. (2009). Culture of endodermal stem/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim, 45(1–2), 44–54. https://doi.org/10.1007/s11626-008-9149-2
Luo, Xiaoyan, Tadashi Okubo, Scott Randell, and Brigid L. M. Hogan. “Culture of endodermal stem/progenitor cells of the mouse tongue.In Vitro Cell Dev Biol Anim 45, no. 1–2 (2009): 44–54. https://doi.org/10.1007/s11626-008-9149-2.
Luo X, Okubo T, Randell S, Hogan BLM. Culture of endodermal stem/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim. 2009;45(1–2):44–54.
Luo, Xiaoyan, et al. “Culture of endodermal stem/progenitor cells of the mouse tongue.In Vitro Cell Dev Biol Anim, vol. 45, no. 1–2, 2009, pp. 44–54. Pubmed, doi:10.1007/s11626-008-9149-2.
Luo X, Okubo T, Randell S, Hogan BLM. Culture of endodermal stem/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim. 2009;45(1–2):44–54.
Journal cover image

Published In

In Vitro Cell Dev Biol Anim

DOI

EISSN

1543-706X

Publication Date

2009

Volume

45

Issue

1-2

Start / End Page

44 / 54

Location

Germany

Related Subject Headings

  • Tongue
  • Taste Buds
  • Stem Cells
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Humans
  • Female
  • Epithelial Cells