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Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes.

Publication ,  Journal Article
Cox, B; Kislinger, T; Wigle, DA; Kannan, A; Brown, K; Okubo, T; Hogan, B; Jurisica, I; Frey, B; Rossant, J; Emili, A
Published in: Mol Syst Biol
2007

Although microarray analysis has provided information regarding the dynamics of gene expression during development of the mouse lung, no extensive correlations have been made to the levels of corresponding protein products. Here, we present a global survey of protein expression during mouse lung organogenesis from embryonic day E13.5 until adulthood using gel-free two-dimensional liquid chromatography coupled to shotgun tandem mass spectrometry (MudPIT). Mathematical modeling of the proteomic profiles with parallel DNA microarray data identified large groups of gene products with statistically significant correlation or divergence in coregulation of protein and transcript levels during lung development. We also present an integrative analysis of mRNA and protein expression in Nmyc loss- and gain-of-function mutants. This revealed a set of 90 positively and negatively regulated putative target genes. These targets are evidence that Nmyc is a regulator of genes involved in mRNA processing and a repressor of the imprinted gene Igf2r in the developing lung.

Duke Scholars

Published In

Mol Syst Biol

DOI

EISSN

1744-4292

Publication Date

2007

Volume

3

Start / End Page

109

Location

Germany

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Subcellular Fractions
  • Recombinant Fusion Proteins
  • Receptor, IGF Type 2
  • RNA, Messenger
  • Proto-Oncogene Proteins c-myc
  • Proteomics
  • Protein Interaction Mapping
  • Pregnancy
 

Citation

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Cox, B., Kislinger, T., Wigle, D. A., Kannan, A., Brown, K., Okubo, T., … Emili, A. (2007). Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes. Mol Syst Biol, 3, 109. https://doi.org/10.1038/msb4100151
Cox, Brian, Thomas Kislinger, Dennis A. Wigle, Anitha Kannan, Kevin Brown, Tadashi Okubo, Brigid Hogan, et al. “Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes.Mol Syst Biol 3 (2007): 109. https://doi.org/10.1038/msb4100151.
Cox B, Kislinger T, Wigle DA, Kannan A, Brown K, Okubo T, et al. Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes. Mol Syst Biol. 2007;3:109.
Cox, Brian, et al. “Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes.Mol Syst Biol, vol. 3, 2007, p. 109. Pubmed, doi:10.1038/msb4100151.
Cox B, Kislinger T, Wigle DA, Kannan A, Brown K, Okubo T, Hogan B, Jurisica I, Frey B, Rossant J, Emili A. Integrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes. Mol Syst Biol. 2007;3:109.
Journal cover image

Published In

Mol Syst Biol

DOI

EISSN

1744-4292

Publication Date

2007

Volume

3

Start / End Page

109

Location

Germany

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Subcellular Fractions
  • Recombinant Fusion Proteins
  • Receptor, IGF Type 2
  • RNA, Messenger
  • Proto-Oncogene Proteins c-myc
  • Proteomics
  • Protein Interaction Mapping
  • Pregnancy