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Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity.

Publication ,  Journal Article
Kohno, T; Wang, H; Amaya, F; Brenner, GJ; Cheng, J-K; Ji, R-R; Woolf, CJ
Published in: J Neurosci
April 23, 2008

Bradykinin potentiates synaptic glutamate release and action in the spinal cord via presynaptic and postsynaptic B(2) receptors, contributing thereby to activity-dependent central sensitization and pain hypersensitivity (Wang et al., 2005). We have now examined the signaling pathways that are responsible for the postsynaptic modulatory actions of bradykinin on glutamatergic action and transmission in superficial dorsal horn neurons. B(2) receptors are coexpressed in dorsal horn neurons with protein kinase A (PKA) and the delta isoform of protein kinase C (PKC), and we find that the augmentation by bradykinin of AMPA and NMDA receptor-mediated currents in lamina II neurons requires coactivation of both PKC and PKA. The activation of PKA is downstream of COX1 (cyclooxygenase-1). Extracellular signal-regulated kinase (ERK) activation is involved after the PKC and PKA coactivation, and intrathecal administration of bradykinin induces a thermal hyperalgesia in vivo, which is reduced by inhibition of ERK, PKA, and PKC. We conclude that bradykinin, by activating multiple kinases in dorsal horn neurons, potentiates glutamatergic synaptic transmission to produce pain hypersensitivity.

Duke Scholars

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

April 23, 2008

Volume

28

Issue

17

Start / End Page

4533 / 4540

Location

United States

Related Subject Headings

  • Spinal Cord
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, AMPA
  • Rats, Sprague-Dawley
  • Rats
  • Protein Kinases
  • Posterior Horn Cells
  • Pain Measurement
  • Pain
  • Neurology & Neurosurgery
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kohno, T., Wang, H., Amaya, F., Brenner, G. J., Cheng, J.-K., Ji, R.-R., & Woolf, C. J. (2008). Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity. J Neurosci, 28(17), 4533–4540. https://doi.org/10.1523/JNEUROSCI.5349-07.2008
Kohno, Tatsuro, Haibin Wang, Fumimasa Amaya, Gary J. Brenner, Jen-Kun Cheng, Ru-Rong Ji, and Clifford J. Woolf. “Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity.J Neurosci 28, no. 17 (April 23, 2008): 4533–40. https://doi.org/10.1523/JNEUROSCI.5349-07.2008.
Kohno T, Wang H, Amaya F, Brenner GJ, Cheng J-K, Ji R-R, et al. Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity. J Neurosci. 2008 Apr 23;28(17):4533–40.
Kohno, Tatsuro, et al. “Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity.J Neurosci, vol. 28, no. 17, Apr. 2008, pp. 4533–40. Pubmed, doi:10.1523/JNEUROSCI.5349-07.2008.
Kohno T, Wang H, Amaya F, Brenner GJ, Cheng J-K, Ji R-R, Woolf CJ. Bradykinin enhances AMPA and NMDA receptor activity in spinal cord dorsal horn neurons by activating multiple kinases to produce pain hypersensitivity. J Neurosci. 2008 Apr 23;28(17):4533–4540.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

April 23, 2008

Volume

28

Issue

17

Start / End Page

4533 / 4540

Location

United States

Related Subject Headings

  • Spinal Cord
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, AMPA
  • Rats, Sprague-Dawley
  • Rats
  • Protein Kinases
  • Posterior Horn Cells
  • Pain Measurement
  • Pain
  • Neurology & Neurosurgery