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Small contribution of G1 checkpoint control manipulation to modulation of p53-mediated apoptosis.

Publication ,  Journal Article
Canman, CE; Kastan, MB
Published in: Oncogene
February 26, 1998

Deregulation of the S-phase promoting E2F-1 transcription factor has been shown to cooperate with p53 to induce apoptosis. BaF3 cells undergo rapid, p53-dependent apoptosis when irradiated in the absence of IL-3. Rapid apoptosis induced by ionizing radiation (IR) coincides with attenuated p21(WAF1/Cip1) induction. Failure to adequately induce p21 could result in inappropriate release of E2F from Rb which may then cooperate with p53 to induce apoptosis in cells deprived of growth factor. We engineered BaF3 cells to express exogenous p21 and tested whether overexpressing p21 in cells irradiated in the absence of IL-3 protects from IR-induced apoptosis. Enforced p21 expression resulted in a consistent, but partial, protection of cells from undergoing IR-induced apoptosis. However, deregulating E2F activity through expression of HPV E7 failed to sensitize cells to IR-induced apoptosis in the presence of IL-3. Together, these data strongly suggest that the IL-3-responsive factors which modulate p53-mediated apoptosis in BaF3 cells are largely independent of G1 cell cycle checkpoint control mediated by p21.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

February 26, 1998

Volume

16

Issue

8

Start / End Page

957 / 966

Location

England

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription Factors
  • Transcription Factor DP1
  • Signal Transduction
  • Retinoblastoma-Binding Protein 1
  • Oncology & Carcinogenesis
  • Mice
  • Interleukin-3
  • Genes, p53
  • G1 Phase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Canman, C. E., & Kastan, M. B. (1998). Small contribution of G1 checkpoint control manipulation to modulation of p53-mediated apoptosis. Oncogene, 16(8), 957–966. https://doi.org/10.1038/sj.onc.1201612
Canman, C. E., and M. B. Kastan. “Small contribution of G1 checkpoint control manipulation to modulation of p53-mediated apoptosis.Oncogene 16, no. 8 (February 26, 1998): 957–66. https://doi.org/10.1038/sj.onc.1201612.
Canman, C. E., and M. B. Kastan. “Small contribution of G1 checkpoint control manipulation to modulation of p53-mediated apoptosis.Oncogene, vol. 16, no. 8, Feb. 1998, pp. 957–66. Pubmed, doi:10.1038/sj.onc.1201612.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

February 26, 1998

Volume

16

Issue

8

Start / End Page

957 / 966

Location

England

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription Factors
  • Transcription Factor DP1
  • Signal Transduction
  • Retinoblastoma-Binding Protein 1
  • Oncology & Carcinogenesis
  • Mice
  • Interleukin-3
  • Genes, p53
  • G1 Phase