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RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.

Publication ,  Journal Article
Mazan-Mamczarz, K; Galbán, S; López de Silanes, I; Martindale, JL; Atasoy, U; Keene, JD; Gorospe, M
Published in: Proc Natl Acad Sci U S A
July 8, 2003

Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 8, 2003

Volume

100

Issue

14

Start / End Page

8354 / 8359

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • RNA-Binding Proteins
  • RNA, Small Interfering
  • RNA, Neoplasm
  • RNA, Messenger
  • Protein Interaction Mapping
  • Protein Biosynthesis
 

Citation

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Mazan-Mamczarz, K., Galbán, S., López de Silanes, I., Martindale, J. L., Atasoy, U., Keene, J. D., & Gorospe, M. (2003). RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A, 100(14), 8354–8359. https://doi.org/10.1073/pnas.1432104100
Mazan-Mamczarz, Krystyna, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, and Myriam Gorospe. “RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.Proc Natl Acad Sci U S A 100, no. 14 (July 8, 2003): 8354–59. https://doi.org/10.1073/pnas.1432104100.
Mazan-Mamczarz K, Galbán S, López de Silanes I, Martindale JL, Atasoy U, Keene JD, et al. RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8354–9.
Mazan-Mamczarz, Krystyna, et al. “RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.Proc Natl Acad Sci U S A, vol. 100, no. 14, July 2003, pp. 8354–59. Pubmed, doi:10.1073/pnas.1432104100.
Mazan-Mamczarz K, Galbán S, López de Silanes I, Martindale JL, Atasoy U, Keene JD, Gorospe M. RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8354–8359.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 8, 2003

Volume

100

Issue

14

Start / End Page

8354 / 8359

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • RNA-Binding Proteins
  • RNA, Small Interfering
  • RNA, Neoplasm
  • RNA, Messenger
  • Protein Interaction Mapping
  • Protein Biosynthesis