RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.
Publication
, Journal Article
Mazan-Mamczarz, K; Galbán, S; López de Silanes, I; Martindale, JL; Atasoy, U; Keene, JD; Gorospe, M
Published in: Proc Natl Acad Sci U S A
July 8, 2003
Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
July 8, 2003
Volume
100
Issue
14
Start / End Page
8354 / 8359
Location
United States
Related Subject Headings
- Ultraviolet Rays
- Tumor Suppressor Protein p53
- Tumor Cells, Cultured
- Transfection
- RNA-Binding Proteins
- RNA, Small Interfering
- RNA, Neoplasm
- RNA, Messenger
- Protein Interaction Mapping
- Protein Biosynthesis
Citation
APA
Chicago
ICMJE
MLA
NLM
Mazan-Mamczarz, K., Galbán, S., López de Silanes, I., Martindale, J. L., Atasoy, U., Keene, J. D., & Gorospe, M. (2003). RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A, 100(14), 8354–8359. https://doi.org/10.1073/pnas.1432104100
Mazan-Mamczarz, Krystyna, Stefanie Galbán, Isabel López de Silanes, Jennifer L. Martindale, Ulus Atasoy, Jack D. Keene, and Myriam Gorospe. “RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.” Proc Natl Acad Sci U S A 100, no. 14 (July 8, 2003): 8354–59. https://doi.org/10.1073/pnas.1432104100.
Mazan-Mamczarz K, Galbán S, López de Silanes I, Martindale JL, Atasoy U, Keene JD, et al. RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8354–9.
Mazan-Mamczarz, Krystyna, et al. “RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.” Proc Natl Acad Sci U S A, vol. 100, no. 14, July 2003, pp. 8354–59. Pubmed, doi:10.1073/pnas.1432104100.
Mazan-Mamczarz K, Galbán S, López de Silanes I, Martindale JL, Atasoy U, Keene JD, Gorospe M. RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8354–8359.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
July 8, 2003
Volume
100
Issue
14
Start / End Page
8354 / 8359
Location
United States
Related Subject Headings
- Ultraviolet Rays
- Tumor Suppressor Protein p53
- Tumor Cells, Cultured
- Transfection
- RNA-Binding Proteins
- RNA, Small Interfering
- RNA, Neoplasm
- RNA, Messenger
- Protein Interaction Mapping
- Protein Biosynthesis