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Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.

Publication ,  Journal Article
Romac, JM; Keene, JD
Published in: Genes Dev
June 1, 1995

Transient transfection of the U1 snRNP 70K protein into COS cells induced nuclear reorganization and redistribution of the splicing factor SC-35, whereas hnRNP proteins were not affected. Correspondingly, splicing and nucleocytoplasmic transport of a coexpressed mRNA substrate was reduced by overexpression of U1-70K. The carboxy-terminal portion of U1-70K-encompassing repeats of Arg/Ser, Arg/Glu, and Arg/Asp localizes to the nucleus independently of U1 RNA and was responsible for these inhibitory effects. This region of U1-70K contains amino acid residues similar to those found in splicing factors SC-35, U2AF, su(wa), and in other SR proteins suggesting that U1-70K protein may serve as a focus of assembly for functional components of the splicing/transport machinery. These findings are compatible with models that propose that direct interaction between U1-70K and SR proteins play a regulatory role in early events of spliceosome assembly.

Duke Scholars

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

June 1, 1995

Volume

9

Issue

11

Start / End Page

1400 / 1410

Location

United States

Related Subject Headings

  • Ribonucleoprotein, U1 Small Nuclear
  • Recombinant Proteins
  • RNA, Messenger
  • RNA Splicing
  • Humans
  • Gene Transfer Techniques
  • Gene Expression Regulation
  • Developmental Biology
  • Cell Line
  • Biological Transport
 

Citation

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Romac, J. M., & Keene, J. D. (1995). Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA. Genes Dev, 9(11), 1400–1410. https://doi.org/10.1101/gad.9.11.1400
Romac, J. M., and J. D. Keene. “Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.Genes Dev 9, no. 11 (June 1, 1995): 1400–1410. https://doi.org/10.1101/gad.9.11.1400.
Romac, J. M., and J. D. Keene. “Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA.Genes Dev, vol. 9, no. 11, June 1995, pp. 1400–10. Pubmed, doi:10.1101/gad.9.11.1400.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

June 1, 1995

Volume

9

Issue

11

Start / End Page

1400 / 1410

Location

United States

Related Subject Headings

  • Ribonucleoprotein, U1 Small Nuclear
  • Recombinant Proteins
  • RNA, Messenger
  • RNA Splicing
  • Humans
  • Gene Transfer Techniques
  • Gene Expression Regulation
  • Developmental Biology
  • Cell Line
  • Biological Transport