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In vitro selection of an RNA epitope immunologically cross-reactive with a peptide.

Publication ,  Journal Article
Tsai, DE; Kenan, DJ; Keene, JD
Published in: Proc Natl Acad Sci U S A
October 1, 1992

An antiserum raised against a peptide was used to select a unique RNA species from a degenerate pool of RNAs designed to resemble an autoantibody recognition site in U1 RNA. The peptide and the selected RNA epitope could compete for antibody binding, suggesting that both RNA and peptide epitopes occupy the same or overlapping antigen-combining sites. Thus, the RNA epitope functioned as a specific inhibitor of the antibody-antigen interaction. We demonstrate that the RNA epitope can be used to tag unrelated RNA molecules and also to detect the presence of the antibody. We propose that sequence-specific recognition of RNA by antibodies may involve protein-RNA contacts similar to those occurring in other nucleic acid-binding proteins. In addition, these findings are compatible with the suggestion that nucleic acid-binding autoantibodies may arise through immunological cross-reactivity between proteins and nucleic acids.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1, 1992

Volume

89

Issue

19

Start / End Page

8864 / 8868

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Restriction Mapping
  • RNA, Small Nuclear
  • RNA
  • Promoter Regions, Genetic
  • Peptides
  • Oligoribonucleotides
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Molecular Sequence Data
 

Citation

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Tsai, D. E., Kenan, D. J., & Keene, J. D. (1992). In vitro selection of an RNA epitope immunologically cross-reactive with a peptide. Proc Natl Acad Sci U S A, 89(19), 8864–8868. https://doi.org/10.1073/pnas.89.19.8864
Tsai, D. E., D. J. Kenan, and J. D. Keene. “In vitro selection of an RNA epitope immunologically cross-reactive with a peptide.Proc Natl Acad Sci U S A 89, no. 19 (October 1, 1992): 8864–68. https://doi.org/10.1073/pnas.89.19.8864.
Tsai DE, Kenan DJ, Keene JD. In vitro selection of an RNA epitope immunologically cross-reactive with a peptide. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8864–8.
Tsai, D. E., et al. “In vitro selection of an RNA epitope immunologically cross-reactive with a peptide.Proc Natl Acad Sci U S A, vol. 89, no. 19, Oct. 1992, pp. 8864–68. Pubmed, doi:10.1073/pnas.89.19.8864.
Tsai DE, Kenan DJ, Keene JD. In vitro selection of an RNA epitope immunologically cross-reactive with a peptide. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8864–8868.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1, 1992

Volume

89

Issue

19

Start / End Page

8864 / 8868

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Restriction Mapping
  • RNA, Small Nuclear
  • RNA
  • Promoter Regions, Genetic
  • Peptides
  • Oligoribonucleotides
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Molecular Sequence Data