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Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.

Publication ,  Journal Article
Kim, N; Huang, S-YN; Williams, JS; Li, YC; Clark, AB; Cho, J-E; Kunkel, TA; Pommier, Y; Jinks-Robertson, S
Published in: Science
June 24, 2011

The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

June 24, 2011

Volume

332

Issue

6037

Start / End Page

1561 / 1564

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Ribonucleotides
  • Ribonuclease H
  • Nucleic Acid Conformation
  • Mutagenesis
  • Molecular Sequence Data
  • Microsatellite Repeats
 

Citation

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Kim, N., Huang, S.-Y., Williams, J. S., Li, Y. C., Clark, A. B., Cho, J.-E., … Jinks-Robertson, S. (2011). Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I. Science, 332(6037), 1561–1564. https://doi.org/10.1126/science.1205016
Kim, Nayun, Shar-yin N. Huang, Jessica S. Williams, Yue C. Li, Alan B. Clark, Jang-Eun Cho, Thomas A. Kunkel, Yves Pommier, and Sue Jinks-Robertson. “Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.Science 332, no. 6037 (June 24, 2011): 1561–64. https://doi.org/10.1126/science.1205016.
Kim N, Huang S-YN, Williams JS, Li YC, Clark AB, Cho J-E, et al. Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I. Science. 2011 Jun 24;332(6037):1561–4.
Kim, Nayun, et al. “Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.Science, vol. 332, no. 6037, June 2011, pp. 1561–64. Pubmed, doi:10.1126/science.1205016.
Kim N, Huang S-YN, Williams JS, Li YC, Clark AB, Cho J-E, Kunkel TA, Pommier Y, Jinks-Robertson S. Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I. Science. 2011 Jun 24;332(6037):1561–1564.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

June 24, 2011

Volume

332

Issue

6037

Start / End Page

1561 / 1564

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Ribonucleotides
  • Ribonuclease H
  • Nucleic Acid Conformation
  • Mutagenesis
  • Molecular Sequence Data
  • Microsatellite Repeats