Dynamic Rheological Studies of Coagulation and Fibrinolysis

A Weissenberg rheogoniometer in the oscillatory mode was employed to determine the effect of fibrinolysis and fibrinogen degradation products (FDP) on the development of the dynamic shear moduli in coagulating platelet-free plasma (PFP) and platelet-rich plasma (PRP). While the FDP produced by incubation of PFP for 3 min with streptokinase (SK) increased the maximum shear storage modulus (G,„/) of PFP, these early FDP decreased the Gmaxof PRP clots significantly. The FDP produced at longer incubation periods reduced the G of PRP clots also. The decreased Gmaj(in PRP containing FDP appears due to FDP inhibiting the binding of polymerizing fibrin to the platelet membrane. While 10 a/ml of SK did not produce FDP or induce significant fibrinolysis, clot lysis was complete and rapid in the presence of 20 u/ml SK. Experiments with crosslinking inhibitors indicate that poorly crosslinked clots are more susceptible to fibrinolysis. In addition, a model for coagulation is presented which accurately fits the entire storage mod ulus-versus-time curve for PFP. © 1979, The Society of Rheology. All rights reserved.

Duke Scholars

Author John P. Kirkpatrick Radiation Oncology