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The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1.

Publication ,  Journal Article
Huang, N-J; Zhang, L; Tang, W; Chen, C; Yang, C-S; Kornbluth, S
Published in: The Journal of cell biology
April 2012

Proapoptotic Bcl-2 family members, such as Bax, promote release of cytochrome c from mitochondria, leading to caspase activation and cell death. It was previously reported that modulator of apoptosis protein 1 (MOAP-1), an enhancer of Bax activation induced by DNA damage, is stabilized by Trim39, a protein of unknown function. In this paper, we show that MOAP-1 is a novel substrate of the anaphase-promoting complex (APC/C(Cdh1)) ubiquitin ligase. The influence of Trim39 on MOAP-1 levels stems from the ability of Trim39 (a RING domain E3 ligase) to directly inhibit APC/C(Cdh1)-mediated protein ubiquitylation. Accordingly, small interfering ribonucleic acid-mediated knockdown of Cdh1 stabilized MOAP-1, thereby enhancing etoposide-induced Bax activation and apoptosis. These data identify Trim39 as a novel APC/C regulator and provide an unexpected link between the APC/C and apoptotic regulation via MOAP-1.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

April 2012

Volume

197

Issue

3

Start / End Page

361 / 367

Related Subject Headings

  • bcl-2-Associated X Protein
  • Ubiquitination
  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Recombinant Proteins
  • RNA, Small Interfering
  • Immunoprecipitation
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, N.-J., Zhang, L., Tang, W., Chen, C., Yang, C.-S., & Kornbluth, S. (2012). The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1. The Journal of Cell Biology, 197(3), 361–367. https://doi.org/10.1083/jcb.201111141
Huang, Nai-Jia, Liguo Zhang, Wanli Tang, Chen Chen, Chih-Sheng Yang, and Sally Kornbluth. “The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1.The Journal of Cell Biology 197, no. 3 (April 2012): 361–67. https://doi.org/10.1083/jcb.201111141.
Huang N-J, Zhang L, Tang W, Chen C, Yang C-S, Kornbluth S. The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1. The Journal of cell biology. 2012 Apr;197(3):361–7.
Huang, Nai-Jia, et al. “The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1.The Journal of Cell Biology, vol. 197, no. 3, Apr. 2012, pp. 361–67. Epmc, doi:10.1083/jcb.201111141.
Huang N-J, Zhang L, Tang W, Chen C, Yang C-S, Kornbluth S. The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1. The Journal of cell biology. 2012 Apr;197(3):361–367.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

April 2012

Volume

197

Issue

3

Start / End Page

361 / 367

Related Subject Headings

  • bcl-2-Associated X Protein
  • Ubiquitination
  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Recombinant Proteins
  • RNA, Small Interfering
  • Immunoprecipitation
  • Humans
  • Hela Cells
  • HeLa Cells