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Cytokine expression by first-trimester human chorionic villi.

Publication ,  Journal Article
Bennett, WA; Lagoo-Deenadayalan, S; Stopple, JA; Barber, WH; Hale, E; Brackin, MN; Cowan, BD
Published in: Am J Reprod Immunol
November 1998

PROBLEM: Communication at the human maternal-fetal interface occurs by an intricate cytokine network. This study examines cytokine expression by normal first-trimester human chorionic villi. METHOD OF STUDY: Tissues were obtained at elective pregnancy terminations (7-9 weeks). Total RNA was isolated from chorionic villi by guanidinium isothiocynate-acid phenol extraction. A reverse transcriptase-polymerase chain reaction technique was used to examine cytokine expression. beta-Actin was used as the housekeeping gene, and mitogen-stimulated lymphocytes served as positive controls. RESULTS: beta-Actin was uniformly expressed by all chorionic villous samples. Interferon (IFN)-alpha and -beta also were highly expressed. Moderate expression was noted for interleukin (IL)-10, IL-6, tumor necrosis factor (TNF)-alpha, and IL-1 beta. In contrast, transforming growth factor-beta 1, IFN-gamma, IL-2, and IL-1 alpha were either weakly expressed or absent in first-trimester villi. CONCLUSIONS: Cytokines may contribute to pregnancy immunotolerance (IFN-alpha, IFN-beta, and IL-10), viral resistance (IFNs), hormone secretion (IL-1 and IL-6), and cellular remodeling (IFN-gamma and TNF-alpha) within the chorionic villous.

Duke Scholars

Published In

Am J Reprod Immunol

DOI

ISSN

1046-7408

Publication Date

November 1998

Volume

40

Issue

5

Start / End Page

309 / 318

Location

Denmark

Related Subject Headings

  • Th1 Cells
  • Reverse Transcriptase Polymerase Chain Reaction
  • Pregnancy Trimester, First
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Inflammation
  • Humans
  • Female
  • Cytokines
  • Chorionic Villi
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bennett, W. A., Lagoo-Deenadayalan, S., Stopple, J. A., Barber, W. H., Hale, E., Brackin, M. N., & Cowan, B. D. (1998). Cytokine expression by first-trimester human chorionic villi. Am J Reprod Immunol, 40(5), 309–318. https://doi.org/10.1111/j.1600-0897.1998.tb00059.x
Bennett, W. A., S. Lagoo-Deenadayalan, J. A. Stopple, W. H. Barber, E. Hale, M. N. Brackin, and B. D. Cowan. “Cytokine expression by first-trimester human chorionic villi.Am J Reprod Immunol 40, no. 5 (November 1998): 309–18. https://doi.org/10.1111/j.1600-0897.1998.tb00059.x.
Bennett WA, Lagoo-Deenadayalan S, Stopple JA, Barber WH, Hale E, Brackin MN, et al. Cytokine expression by first-trimester human chorionic villi. Am J Reprod Immunol. 1998 Nov;40(5):309–18.
Bennett, W. A., et al. “Cytokine expression by first-trimester human chorionic villi.Am J Reprod Immunol, vol. 40, no. 5, Nov. 1998, pp. 309–18. Pubmed, doi:10.1111/j.1600-0897.1998.tb00059.x.
Bennett WA, Lagoo-Deenadayalan S, Stopple JA, Barber WH, Hale E, Brackin MN, Cowan BD. Cytokine expression by first-trimester human chorionic villi. Am J Reprod Immunol. 1998 Nov;40(5):309–318.
Journal cover image

Published In

Am J Reprod Immunol

DOI

ISSN

1046-7408

Publication Date

November 1998

Volume

40

Issue

5

Start / End Page

309 / 318

Location

Denmark

Related Subject Headings

  • Th1 Cells
  • Reverse Transcriptase Polymerase Chain Reaction
  • Pregnancy Trimester, First
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Inflammation
  • Humans
  • Female
  • Cytokines
  • Chorionic Villi