
Functional reconstitution of purified human Hv1 H+ channels.
Publication
, Journal Article
Lee, S-Y; Letts, JA; MacKinnon, R
Published in: J Mol Biol
April 17, 2009
Voltage-dependent H(+) (Hv) channels mediate proton conduction into and out of cells under the control of membrane voltage. Hv channels are unusual compared to voltage-dependent K(+), Na(+), and Ca(2+) channels in that Hv channel genes encode a voltage sensor domain (VSD) without a pore domain. The H(+) currents observed when Hv channels are expressed heterologously suggest that the VSD itself provides the pathway for proton conduction. In order to exclude the possibility that the Hv channel VSD assembles with an as yet unknown protein in the cell membrane as a requirement for H(+) conduction, we have purified Hv channels to homogeneity and reconstituted them into synthetic lipid liposomes. The Hv channel VSD by itself supports H(+) flux.
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Published In
J Mol Biol
DOI
EISSN
1089-8638
Publication Date
April 17, 2009
Volume
387
Issue
5
Start / End Page
1055 / 1060
Location
Netherlands
Related Subject Headings
- Recombinant Proteins
- Protons
- Liposomes
- Ion Channels
- In Vitro Techniques
- Hydrogen-Ion Concentration
- Humans
- Fluorescence
- Electrochemistry
- Biochemistry & Molecular Biology
Citation
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Lee, S.-Y., Letts, J. A., & MacKinnon, R. (2009). Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol, 387(5), 1055–1060. https://doi.org/10.1016/j.jmb.2009.02.034
Lee, Seok-Yong, James A. Letts, and Roderick MacKinnon. “Functional reconstitution of purified human Hv1 H+ channels.” J Mol Biol 387, no. 5 (April 17, 2009): 1055–60. https://doi.org/10.1016/j.jmb.2009.02.034.
Lee S-Y, Letts JA, MacKinnon R. Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol. 2009 Apr 17;387(5):1055–60.
Lee, Seok-Yong, et al. “Functional reconstitution of purified human Hv1 H+ channels.” J Mol Biol, vol. 387, no. 5, Apr. 2009, pp. 1055–60. Pubmed, doi:10.1016/j.jmb.2009.02.034.
Lee S-Y, Letts JA, MacKinnon R. Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol. 2009 Apr 17;387(5):1055–1060.

Published In
J Mol Biol
DOI
EISSN
1089-8638
Publication Date
April 17, 2009
Volume
387
Issue
5
Start / End Page
1055 / 1060
Location
Netherlands
Related Subject Headings
- Recombinant Proteins
- Protons
- Liposomes
- Ion Channels
- In Vitro Techniques
- Hydrogen-Ion Concentration
- Humans
- Fluorescence
- Electrochemistry
- Biochemistry & Molecular Biology