Crystal structure of an activated response regulator bound to its target.
Publication
, Journal Article
Lee, SY; Cho, HS; Pelton, JG; Yan, D; Henderson, RK; King, DS; Huang, L; Kustu, S; Berry, EA; Wemmer, DE
Published in: Nat Struct Biol
January 2001
The chemotactic regulator CheY controls the direction of flagellar rotation in Escherichia coli. We have determined the crystal structure of BeF3--activated CheY from E. coli in complex with an N-terminal peptide derived from its target, FliM. The structure reveals that the first seven residues of the peptide pack against the beta4-H4 loop and helix H4 of CheY in an extended conformation, whereas residues 8-15 form two turns of helix and pack against the H4-beta5-H5 face. The peptide binds the only region of CheY that undergoes noticeable conformational change upon activation and would most likely be sandwiched between activated CheY and the remainder of FliM to reverse the direction of flagellar rotation.
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Published In
Nat Struct Biol
DOI
ISSN
1072-8368
Publication Date
January 2001
Volume
8
Issue
1
Start / End Page
52 / 56
Location
United States
Related Subject Headings
- Static Electricity
- Sequence Alignment
- Rotation
- Protein Structure, Secondary
- Peptide Fragments
- Molecular Sequence Data
- Models, Molecular
- Methyl-Accepting Chemotaxis Proteins
- Membrane Proteins
- Fluorides
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Lee, S. Y., Cho, H. S., Pelton, J. G., Yan, D., Henderson, R. K., King, D. S., … Wemmer, D. E. (2001). Crystal structure of an activated response regulator bound to its target. Nat Struct Biol, 8(1), 52–56. https://doi.org/10.1038/83053
Lee, S. Y., H. S. Cho, J. G. Pelton, D. Yan, R. K. Henderson, D. S. King, L. Huang, S. Kustu, E. A. Berry, and D. E. Wemmer. “Crystal structure of an activated response regulator bound to its target.” Nat Struct Biol 8, no. 1 (January 2001): 52–56. https://doi.org/10.1038/83053.
Lee SY, Cho HS, Pelton JG, Yan D, Henderson RK, King DS, et al. Crystal structure of an activated response regulator bound to its target. Nat Struct Biol. 2001 Jan;8(1):52–6.
Lee, S. Y., et al. “Crystal structure of an activated response regulator bound to its target.” Nat Struct Biol, vol. 8, no. 1, Jan. 2001, pp. 52–56. Pubmed, doi:10.1038/83053.
Lee SY, Cho HS, Pelton JG, Yan D, Henderson RK, King DS, Huang L, Kustu S, Berry EA, Wemmer DE. Crystal structure of an activated response regulator bound to its target. Nat Struct Biol. 2001 Jan;8(1):52–56.
Published In
Nat Struct Biol
DOI
ISSN
1072-8368
Publication Date
January 2001
Volume
8
Issue
1
Start / End Page
52 / 56
Location
United States
Related Subject Headings
- Static Electricity
- Sequence Alignment
- Rotation
- Protein Structure, Secondary
- Peptide Fragments
- Molecular Sequence Data
- Models, Molecular
- Methyl-Accepting Chemotaxis Proteins
- Membrane Proteins
- Fluorides