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Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.

Publication ,  Journal Article
Rosoklija, G; Mancevski, B; Ilievski, B; Perera, T; Lisanby, SH; Coplan, JD; Duma, A; Serafimova, T; Dwork, AJ
Published in: J Neurosci Methods
December 30, 2003

Golgi impregnation is unique in its ability to display the dendritic trees of large numbers of individual neurons. However, its reputation for inconsistency leaves many investigators reluctant to embrace this methodology, particularly for the study of formalin-fixed human brain tissue. After reviewing the literature, testing a variety of technical variations, and discussing the procedure with experienced practitioners, we have concluded that much of the unpredictability can be removed by matching the Golgi technique to the conditions that were used for fixation of the tissue. Briefly fixed tissues worked best with the rapid Golgi technique, which includes osmium during the initial chromation step, and with the Golgi-Cox method, which includes mercuric chloride during chromation. For tissues that have been fixed for several years or even for several decades, superior results are obtained with the Golgi-Kopsch technique, using multiple changes of a chromation solution that contains paraformaldehyde. In the Golgi-Kopsch technique, pH should be used to monitor the reduction of Cr6+ to Cr3+, which is a crucial determinant of successful chromation. With any Golgi technique, agitation throughout the impregnation helps to avoid precipitates and to improve the quality of impregnation. When the appropriate method is chosen, Golgi impregnation is a useful technique for the neuropathologist.

Duke Scholars

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

December 30, 2003

Volume

131

Issue

1-2

Start / End Page

1 / 7

Location

Netherlands

Related Subject Headings

  • Tissue Fixation
  • Time Factors
  • Silver Staining
  • Neurology & Neurosurgery
  • Humans
  • Hippocampus
  • Haplorhini
  • Cerebral Cortex
  • Animals
  • 3209 Neurosciences
 

Citation

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Rosoklija, G., Mancevski, B., Ilievski, B., Perera, T., Lisanby, S. H., Coplan, J. D., … Dwork, A. J. (2003). Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys. J Neurosci Methods, 131(1–2), 1–7. https://doi.org/10.1016/j.jneumeth.2003.06.001
Rosoklija, Gorazd, Branislav Mancevski, Boro Ilievski, Tarique Perera, Sarah H. Lisanby, Jeremy D. Coplan, Aleksej Duma, Tereza Serafimova, and Andrew J. Dwork. “Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.J Neurosci Methods 131, no. 1–2 (December 30, 2003): 1–7. https://doi.org/10.1016/j.jneumeth.2003.06.001.
Rosoklija G, Mancevski B, Ilievski B, Perera T, Lisanby SH, Coplan JD, et al. Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys. J Neurosci Methods. 2003 Dec 30;131(1–2):1–7.
Rosoklija, Gorazd, et al. “Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys.J Neurosci Methods, vol. 131, no. 1–2, Dec. 2003, pp. 1–7. Pubmed, doi:10.1016/j.jneumeth.2003.06.001.
Rosoklija G, Mancevski B, Ilievski B, Perera T, Lisanby SH, Coplan JD, Duma A, Serafimova T, Dwork AJ. Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys. J Neurosci Methods. 2003 Dec 30;131(1–2):1–7.
Journal cover image

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

December 30, 2003

Volume

131

Issue

1-2

Start / End Page

1 / 7

Location

Netherlands

Related Subject Headings

  • Tissue Fixation
  • Time Factors
  • Silver Staining
  • Neurology & Neurosurgery
  • Humans
  • Hippocampus
  • Haplorhini
  • Cerebral Cortex
  • Animals
  • 3209 Neurosciences