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Progressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity.

Publication ,  Journal Article
Macklis, JD; Madison, RD
Published in: J Neurosci Methods
January 1990

We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We correlate the progressive loss of resting membrane potential and the progressive inability to generate stimulated action potentials by cultured mouse dorsal root ganglion neurons with increasing incorporation of PI. The scoring system used to gauge incorporation of PI is rapid and highly reproducible using a standard fluorescence microscope. Applications exist for studies of neuronal toxicity, survival, and electrophysiology in vitro.

Duke Scholars

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Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

January 1990

Volume

31

Issue

1

Start / End Page

43 / 46

Location

Netherlands

Related Subject Headings

  • Propidium
  • Phenanthridines
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Membrane Potentials
  • Ganglia, Spinal
  • Fluorescent Dyes
  • Cells, Cultured
  • Cell Membrane
 

Citation

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ICMJE
MLA
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Journal cover image

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

January 1990

Volume

31

Issue

1

Start / End Page

43 / 46

Location

Netherlands

Related Subject Headings

  • Propidium
  • Phenanthridines
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Membrane Potentials
  • Ganglia, Spinal
  • Fluorescent Dyes
  • Cells, Cultured
  • Cell Membrane