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Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis.

Publication ,  Journal Article
Macklis, JD; Madison, R
Published in: Brain Res
December 16, 1985

Selective photothermolysis (SP) is a novel technique by which brief, unfocused laser pulses are selectively absorbed by, and cause selective thermal damage to, endogenously pigmented structures. The present experiments demonstrate the feasibility of using an exogenous non-fluorescent chromophore (procion blue) to effect cellular damage by SP. Dorsal root ganglia neurons in vitro were selectively labeled with procion blue and subsequently damaged by unfocused laser illumination. Progressive cellular damage was assessed by propidium iodide (PI), a fluorescent dye that leaks through damaged membranes and binds to nucleic acids. Graded scores of intracellular PI fluorescence demonstrated a highly significant difference in amount of damage between groups of experimental and control cells. Selective photothermolysis is discussed as an experimental tool for neurobiologists in particular and for general use within the biomedical field.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

December 16, 1985

Volume

359

Issue

1-2

Start / End Page

158 / 165

Location

Netherlands

Related Subject Headings

  • Wheat Germ Agglutinins
  • Triazines
  • Neurology & Neurosurgery
  • Neurobiology
  • Nervous System
  • Mice, Inbred C57BL
  • Mice
  • Lectins
  • Lasers
  • Hyperthermia, Induced
 

Citation

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Macklis, J. D., & Madison, R. (1985). Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis. Brain Res, 359(1–2), 158–165. https://doi.org/10.1016/0006-8993(85)91424-6
Macklis, J. D., and R. Madison. “Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis.Brain Res 359, no. 1–2 (December 16, 1985): 158–65. https://doi.org/10.1016/0006-8993(85)91424-6.
Macklis JD, Madison R. Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis. Brain Res. 1985 Dec 16;359(1–2):158–65.
Macklis, J. D., and R. Madison. “Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis.Brain Res, vol. 359, no. 1–2, Dec. 1985, pp. 158–65. Pubmed, doi:10.1016/0006-8993(85)91424-6.
Macklis JD, Madison R. Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis. Brain Res. 1985 Dec 16;359(1–2):158–165.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

December 16, 1985

Volume

359

Issue

1-2

Start / End Page

158 / 165

Location

Netherlands

Related Subject Headings

  • Wheat Germ Agglutinins
  • Triazines
  • Neurology & Neurosurgery
  • Neurobiology
  • Nervous System
  • Mice, Inbred C57BL
  • Mice
  • Lectins
  • Lasers
  • Hyperthermia, Induced